Rabbit anti-Goat Light Chain Secondary Antibody

Purified light chains isolated from polyclonal goat IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, but a wide scale of reactivity with IgG of other mammalian species must be assumed. The degree of cross-reactivity depends in addition to the phylogenetic relationship also on the concentrations of the reactants and the sensitivity of the assay format.

As tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal goat serum and purified IgG fractions and light chain preparations, the antiserum reacts with determinants exposed on the surface (SD) and the Fab portion of all intact immunoglobulins, with Fab or F(ab')2 subunits, and with free light chains of both types. This antiserum is not kappa or lambda chain specific. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Polyclonal

Rabbit

The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product

Whole Antiserum

Reconstitute by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2).

Whole antiserum

No Preservative

LYOPH mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.