Lin28 1632

Tocris Bioscience | Catalog # 6068

RNA binding protein Lin28 inhibitor; promotes mESC differentiation; also bromodomain inhibitor
Tocris Bioscience
Discontinued Product
6068 has been discontinued. View all DNA, RNA and Protein Synthesis Inhibitors products.

Key Product Details

Description

RNA binding protein Lin28 inhibitor; promotes mESC differentiation; also bromodomain inhibitor
View all DNA, RNA and Protein Synthesis Inhibitors »

Product Description

Lin28 1632 is an RNA binding protein Lin28 inhibitor (IC50 = 8 μM). Lin28 1632 blocks binding of Lin28 to let-7 pre-micro RNA. Lin28 1632 inhibits stemness and induces differentiation of murine ESCs. Lin28 1632 increases let-7 miRNA and suppresses PD-L1 expression in multiple human cancer cell lines. Lin28 1632 inhibits growth of tumor cell lines and tumor-sphere formation in vitro. Lin28 1632 also inhibits growth of tumor xenografts in mice by enhancing T cell-mediated antitumor immunity. Lin28 1632 is also bromodomain inhibitor. Lin28 1632 inhibits tumor growth and metastasis in vivo in immunodeficient orthotopic mouse models of human breast cancer cell MDA-MB-231.

Product Specifications for Lin28 1632

Molecular Weight

281.31

Formula

C15H15N5O

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

N-Methyl-N-[3-(3-methyl-1,2,4-triazolo[4,3-b]pyridazin-6-yl)phenyl]acetamide

CAS Number

108825-65-6

PubChem ID

2737312

InChI Key

WPAQLESUVYGUJZ-UHFFFAOYSA-N

SMILES

CN(C1=CC=CC(C2=NN3C(C)=NN=C3C=C2)=C1)C(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.13 100
Ethanol 28.13 100

Preparing Stock Solutions for Lin28 1632

The following data is based on the product molecular weight 281.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.55 mL 17.77 mL 35.55 mL
5 mM 0.71 mL 3.55 mL 7.11 mL
10 mM 0.36 mL 1.78 mL 3.55 mL
50 mM 0.07 mL 0.36 mL 0.71 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.

Product Documents for Lin28 1632

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lin28 1632

For research use only

Customer Reviews for Lin28 1632 (1)

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  • Good inhibitor for Lin28
    Name: Satbir Thakur
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: YP-MEL, T47D and BT183
    Verified Customer | Posted 05/17/2021
    Used as an inhibitor for Lin28 for 96 h in cell lines expressing Lin28. The protein level was then estimated using a western blot method.
    Lin28 1632 6068

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