Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)
Novus Biologicals | Catalog # NB820-59242
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Key Product Details
Species
Human
Applications
Western Blot
Product Summary for Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
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Product Specifications
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Specificity
Total Protein - Human Adult Normal Tissue: Lymph Node
Type
Tissue
Life Stage
Adult
Tissue
Blood Lymph
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole
Scientific Data Images for Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)
Western Blot: Human Lymph Node Whole Tissue Lysate (Adult Whole Normal) [NB820-59242]
Western Blot: Human Lymph Node Whole Tissue Lysate (Adult Whole Normal) [NB820-59242] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.Formulation, Preparation, and Storage
Formulation
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: Lymph Node
Additional Lymph Node Products
Product Documents for Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)
Product Specific Notices for Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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