MAD1L1/MAD1 Antibody (JE35-54)

Novus Biologicals | Catalog # NBP3-32556

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE35-54 expressed in HEK293
View all MAD1L1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human MAD1L1/MAD1 aa 31-180 / 718. (Uniprot: Q9Y6D9)

Localization

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAD1L1/MAD1 Antibody (JE35-54)

MAD1L1/MAD1 Antibody (JE35-54)

Western Blot: MAD1L1/MAD1 Antibody (JE35-54) [NBP3-32556]

Western Blot: MAD1L1/MAD1 Antibody (JE35-54) [NBP3-32556] - Western blot analysis of MAD1L1/MAD1 on different lysates with Rabbit anti-MAD1L1/MAD1 antibody (NBP3-32556) at 1/1,000 dilution.

Lane 1: HCT 116 cell lysate
Lane 2: HeLa cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: Jurkat cell lysate
Lane 5: MCF7 cell lysate
Lane 6: A431 cell lysate
Lane 7: A375 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 83 kDa
Observed band size: 83 kDa

Exposure time: 15 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32556) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for MAD1L1/MAD1 Antibody (JE35-54)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAD1L1

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.

Long Name

MAD1 Mitotic Arrest Deficient-Like 1

Alternate Names

HsMAD1, MAD1, PIG9, TP53I9, TXBP181

Gene Symbol

MAD1L1

Additional MAD1L1 Products

Product Documents for MAD1L1/MAD1 Antibody (JE35-54)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MAD1L1/MAD1 Antibody (JE35-54)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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