MEKK4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32187

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MEKK4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 75 to 125 of human Mitogen-Activated Protein Kinase Kinase Kinase 4 using the numbering given in entry NP_005913.2 (GeneID 4216).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEKK4 Antibody - BSA Free

Western Blot: MEKK4 Antibody [NBP2-32187]

Western Blot: MEKK4 Antibody [NBP2-32187]

Western Blot: MEKK4 Antibody [NBP2-32187] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells. Antibodies: Affinity purified rabbit anti-MEKK4 antibody NBP2-32187 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: MEKK4 Antibody [NBP2-32187]

Immunoprecipitation: MEKK4 Antibody [NBP2-32187]

Immunoprecipitation: MEKK4 Antibody [NBP2-32187] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-MEKK4 antibody NBP2-32187 used for IP at 6 ug per reaction. MEKK4 was also immunoprecipitated by rabbit anti-MEKK4 antibody BL15818. For blotting immunoprecipitated MEKK4, NBP2-32187 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for MEKK4 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MEKK4

MEK kinase 4 (MEKK4), also known as MTK1, is a Ser/Thr protein kinase belonging to the MAP kinase kinase kinase family (1). This protein contains a protein kinase catalytic domain at the C-terminus. The N-terminal, non-kinase domain may contain a regulatory domain. MEKK4 regulates p38 and JNK, butnot ERK pathway, through the phosphorylation of the MAP2Ks MKK3 and MKK6 and MKK4 and MKK7 respectively (1-2).

Alternate Names

EC 2.7.11.25, FLJ42439, MAPKKK4yeast, homolog of, MEKK 4, mitogen-activated protein kinase kinase kinase 4

Gene Symbol

MAP3K4

UniProt

NP_005913.2 (Human)

Additional MEKK4 Products

Product Documents for MEKK4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MEKK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MEKK4 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review MEKK4 Antibody - BSA Free and earn rewards!

Have you used MEKK4 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies