Methoxy-X04

Tocris Bioscience | Catalog # 4920

Brain penetrant fluorescent amyloid β probe. Used for: detection and quantification of plaques, tangles and cerebrovascular amyloid. Application: confocal microscopy, suitable for in vivo imaging
Tocris Bioscience

Key Product Details

Description

Brain penetrant fluorescent amyloid β probe. Used for: detection and quantification of plaques, tangles and cerebrovascular amyloid. Application: confocal microscopy, suitable for in vivo imaging

Wavelength

Excitation = 370 nm, Emission = 452 nm
View all Fluorescent Amyloid B Probes »

Product Description

Key information: Methoxy-X04 is a blood-brain barrier penetrant fluorescent amyloid β (Aβ) probe. Suitable for in vivo imaging.

Used for: the detection and quantification of Aβ plaques, tangles and cerebrovascular amyloid. Gives high resolution fluorescent imaging on individual plaques in live animals by 2-photon microscopy. Methoxy-X04 co-localizes with CD68+ phagosomes in plaque-associated Iba1+ microglia; and it labels molecularly distinct plaque-associated microglia populations.

Application: confocal microscopy, in vivo imaging.

Properties and Photophysical Data: Methoxy-X04 displays high in vitro binding affinity (Ki = 26.8 nM); binds selectively to fibrillar β-sheet deposits. Excitation and emission maxima (λ) are 370 nm and 452 nm, respectively.

Optical Data

λabs 370 nm
λem 452 nm

Product Specifications for Methoxy-X04

Molecular Weight

344.40

Formula

C23H20O3

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

4,4'-[(2-methoxy-1,4-phenylene)di-(1E)-2,1-ethenediyl]bisphenol

CAS Number

863918-78-9

PubChem ID

16049314

InChI Key

FGYNZFHVGOFCMD-KHVHPYDTSA-N

SMILES

OC(C=C3)=CC=C3/C=C/C1=CC(OC)=C(/C=C/C2=CC=C(O)C=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 34.44 100
Ethanol 3.44 10

Preparing Stock Solutions for Methoxy-X04

The following data is based on the product molecular weight 344.40.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.90 mL 14.52 mL 29.04 mL
5 mM 0.58 mL 2.90 mL 5.81 mL
10 mM 0.29 mL 1.45 mL 2.90 mL
50 mM 0.06 mL 0.29 mL 0.58 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 165 publications citing the usage of this product.

Product Documents for Methoxy-X04

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Methoxy-X04

For research use only

Customer Reviews for Methoxy-X04 (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

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  • Great product for quantifying plaques in tissue sections or studying microglial phagocytosis
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: Brain
    Verified Customer | Posted 09/05/2025
    IP injected mice two hours before harvesting brain. Amyloid clearly labelled when doing fluorescent microscopy, but also great product to assess microglial phagocytosis of amyloid by FACS. Blue is Methoxy and green is anti-amyloid antibody staining
    Methoxy-X04 4920
  • Methoxy staining of amyloid plaques in mouse brain
    Name: Cody Zurhellen
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Brain
    Verified Customer | Posted 04/24/2023
    Methoxy staining of mouse brain (hippocampus) section mounted on glass microscope slide.
    Methoxy-X04 4920
  • Methoxy staining for hippocampal slices
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Verified Customer | Posted 02/05/2020
    The hippocampal slices from mice brain was dyed with methoxy X04 in PBS-EtOH solution. The slices were kept in dark and were then mounted with dako vector mounting media
    Methoxy-X04 4920
  • Amyloid plaque labelling
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vivo
    Verified Customer | Posted 11/19/2018
    Injected IV, Methoxy-X04 labels amyloid plaques in AD models. Great tool to image various cellular processes around AD plaques.

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