Mitomycin C

Tocris Bioscience | Catalog # 3258

DNA cross-linking antitumor agent
Tocris Bioscience

Key Product Details

Description

DNA cross-linking antitumor agent

Alternative Names

Ametycine
View all Apoptosis Inducer Compounds »

Product Description

Mitomycin C is an antibiotic and antitumor agent. Covalently binds DNA forming intra- and interstrand crosslinks. Inhibits DNA synthesis. Also used for MEF/STO feeder layer preparation in stem cell culture.

Product Specifications for Mitomycin C

Molecular Weight

334.33

Formula

C15H18N4O5

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

[1aS-(1aα,8β,8aα,8bα)]-6-Amino-8-[[(aminocarbonyl)oxy]methyl]-1,1a,2,8,8a,8b-hexahydro-8a-methoxy-5-methylazirino[2',3':3,4]pyrrolo[1,2-a]indole-4,7-dione

CAS Number

50-07-7

PubChem ID

5746

InChI Key

NWIBSHFKIJFRCO-WUDYKRTCSA-N

SMILES

O=C(C(N)=C1C)C2=C(N(C[C@H]4[C@@H]3N4)[C@@]3(OC)[C@@H]2COC(N)=O)C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 0.33 1
DMSO 33.43 100

Preparing Stock Solutions for Mitomycin C

The following data is based on the product molecular weight 334.33.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.99 mL 14.96 mL 29.91 mL
5 mM 0.60 mL 2.99 mL 5.98 mL
10 mM 0.30 mL 1.50 mL 2.99 mL
50 mM 0.06 mL 0.30 mL 0.60 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 89 publications citing the usage of this product.

Product Documents for Mitomycin C

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mitomycin C

For research use only

⚠ WARNING: This product can expose you to chemicals including Mitomycin C, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov

Customer Reviews for Mitomycin C (2)

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  • Works to stop HDF proliferation.
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HDF
    Verified Customer | Posted 07/25/2025
    Used to inactivate fibroblasts.
  • itotically inactivates mouse embryonic fibroblasts (MEFs) inactivated by Mitomycin treatment
    Name: Harry Clarke
    Species: Human
    Verified Customer | Posted 02/03/2019
    For studying cell cycle arrest Mitomycin has been used. MEFs have been inactivated with Mitomycin treatment.
    Mitomycin C 3258

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