Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Cell Culture, In vivo assay, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Axl Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Axl
His20-Pro443
Accession # Q80YQ3

Specificity

Detects mouse Axl in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Axl is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Axl Antibody

Axl antibody in Mouse Kidney by Immunohistochemistry (IHC-P).

Axl in Mouse Kidney.

Axl was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Mouse Axl by Immunohistochemistry

Detection of Mouse Axl by Immunohistochemistry

Renal microvascular expression of Axl, Fyn, and Lck in the kidney in unstimulated control and TNF-alpha -exposed mice. Images of kidney cryosections of mice exposed to 200 ng TNF-alpha for 2 h showing localisation of (A) Axl (B) Fyn and (C) Lck. Representative images are shown. Arrowheads indicate different microvascular beds, arterioles (A), glomeruli (g), peritubular capillaries (ptc), and venules (v). Original magnification is ×200. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36532777), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Axl by Immunohistochemistry

Detection of Mouse Axl by Immunohistochemistry

Renal microvascular expression of Axl, Fyn, and Lck in the kidney in unstimulated control and TNF-alpha -exposed mice. Images of kidney cryosections of mice exposed to 200 ng TNF-alpha for 2 h showing localisation of (A) Axl (B) Fyn and (C) Lck. Representative images are shown. Arrowheads indicate different microvascular beds, arterioles (A), glomeruli (g), peritubular capillaries (ptc), and venules (v). Original magnification is ×200. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36532777), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Axl by Western Blot

Detection of Mouse Axl by Western Blot

Kinase presence and activity in TNF-alpha -stimulated HUVEC. The influence of different durations of TNF-alpha (10 ng/ml) stimulation on the phosphorylation and total protein expression of (A) p65 and (B) ERK1/2 determined in HUVEC by immunoblotting. Quantification and normalisation of phosphorylated protein to total protein is shown as fold-change to unstimulated control. (C) Selection of three kinases as potential targets based on time-course data of the mean specificity score and mean kinase statistic (see Materials and Methods). The mean specificity score is based on peptide phosphorylation patterns relative to the unstimulated control condition and represents the likelihood of a kinase to be present (gradation of red). The mean kinase statistic indicates the predicted activity of the kinase relative to untreated control (gradation from blue, less active, to red, highly active). (D) Immunoblot of total HUVEC protein lysates showing presence of Axl and Fyn in control conditions or following 2 h TNF-alpha stimulation in HUVE. (E) Immunoblot of total HUVEC protein lysates showing absence of Lck in control conditions or following 2 h TNF-alpha stimulation in HUVEC. n = 3 independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36532777), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Axl by Western Blot

Detection of Mouse Axl by Western Blot

Kinase presence and activity in TNF-alpha -stimulated HUVEC. The influence of different durations of TNF-alpha (10 ng/ml) stimulation on the phosphorylation and total protein expression of (A) p65 and (B) ERK1/2 determined in HUVEC by immunoblotting. Quantification and normalisation of phosphorylated protein to total protein is shown as fold-change to unstimulated control. (C) Selection of three kinases as potential targets based on time-course data of the mean specificity score and mean kinase statistic (see Materials and Methods). The mean specificity score is based on peptide phosphorylation patterns relative to the unstimulated control condition and represents the likelihood of a kinase to be present (gradation of red). The mean kinase statistic indicates the predicted activity of the kinase relative to untreated control (gradation from blue, less active, to red, highly active). (D) Immunoblot of total HUVEC protein lysates showing presence of Axl and Fyn in control conditions or following 2 h TNF-alpha stimulation in HUVE. (E) Immunoblot of total HUVEC protein lysates showing absence of Lck in control conditions or following 2 h TNF-alpha stimulation in HUVEC. n = 3 independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36532777), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Axl by Immunohistochemistry

Detection of Axl by Immunohistochemistry

Elevated AXL Protein Levels in HN and T2DN Tissues and Serum. (a) AXL immunohistochemistry staining. (b) Ratio of strong positive pixels to total section area. (c) ELISA results for soluble AXL in serum from HN and control patients (n = 13 for HN, n = 14 for control). (d) ELISA results for serum from T2DN/T1DN and control patients (n = 7 for T2DN, n = 2 for T1DN, n = 9 for control). Measurements are presented in ng/mL. p‐values from ImageScope analyses are based on the Kruskal–Wallis test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37813528), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Axl Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse kidney

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Axl Fc Chimera (Catalog # 854-AX)

Reviewed Applications

Read 7 reviews rated 4 using AF854 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References

  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Long Name

Axl Receptor Tyrosine Kinase

Alternate Names

AI323647, ARK, JTK11, Tyro7, UFO

Entrez Gene IDs

558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)

Gene Symbol

AXL

UniProt

Q80YQ3

Additional Axl Products

Product Documents for Mouse Axl Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Axl Antibody

For research use only

Citations for Mouse Axl Antibody

Customer Reviews for Mouse Axl Antibody (7)

4 out of 5
7 Customer Ratings
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Customer Images

Showing  1 - 5 的 7 reviews Showing All
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  • Mouse Axl Antibody
    Name: Tara Peters
    Application: Immunohistochemistry
    Sample Tested: Cancer Tissue
    Species: Mouse
    Verified Customer | Posted 11/19/2019
    Stained FFPE tissue sections from mouse lung tumors with 1:20 and 1:100 dilutions of AF854, detected with DAB chromagen.
    Mouse Axl Antibody AF854
  • Mouse Axl Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen mouse spleen
    Species: Mouse
    Verified Customer | Posted 04/12/2019
    Immunofluorescence of fresh-frozen mouse spleen stained with Axl 1:100
    Mouse Axl Antibody AF854
  • Mouse Axl Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: B16-F1 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 02/19/2019
    The first lane is a wild type line, and the next to are shRNA Axl knockdowns
    Mouse Axl Antibody AF854
  • Mouse Axl Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 01/04/2019
  • Mouse Axl Antibody
    Name: Marco Onorati
    Application: Immunohistochemistry
    Sample Tested: Brain (cerebral cortex)
    Species: Mouse
    Verified Customer | Posted 06/22/2017
  • Mouse Axl Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Spinal cord and Brain tissue
    Species: Mouse
    Verified Customer | Posted 06/07/2017
  • Mouse Axl Antibody
    Name: Juwen DuBois
    Application: in vivo
    Sample Tested: brain and spinal cord
    Species: Mouse
    Verified Customer | Posted 04/03/2017
    Used in vivo to activate Axl in the CNS.

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