Mouse Bcl‑2 related protein A1 Antibody

R&D Systems | Catalog # MAB851

R&D Systems
Discontinued Product
MAB851 has been discontinued. View all Bcl-2 related protein A1 products.

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 78616
View all Bcl-2 related protein A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Bcl‑2 related protein A1
Met1-Ser152
Accession # Q07440

Specificity

Detects mouse Bcl‑2 related protein A1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Bcl‑2 related protein A1 Antibody

Detection of Mouse Bcl‑2 related protein A1 antibody by Western Blot.

Detection of Mouse Bcl‑2 related protein A1 by Western Blot.

Western blot shows samples of Recombinant Human Bcl-2 related protein A1 Minus C-terminus, aa 1-152 (Catalog # 1160-A1) (0.3 ng and 1 ng). PVDF membrane was probed with 1 µg/mL Rat Anti-Mouse Bcl-2 related protein A1 Monoclonal Antibody (Catalog # MAB851) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for Bcl-2 related protein A1 was detected at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Mouse Bcl‑2 related protein A1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Bcl‑2 related protein A1 Minus C-terminus, aa 1-152 (Catalog # 1160-A1)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bcl-2 related protein A1

Bcl-2 related protein A1 (abbreviated Bcl2a1 or A1) is an anti-apoptotic outer mitochondrial membrane protein that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. A1 prevents apoptosis by binding to the BH3 region of pro-apoptotic Bcl-2 family members.

Alternate Names

BCL2A1, Bcl2L5, Bfl-1, GRS

Entrez Gene IDs

597 (Human); 12044 (Mouse)

Gene Symbol

BCL2A1

UniProt

Q07440

Additional Bcl-2 related protein A1 Products

Product Documents for Mouse Bcl‑2 related protein A1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Bcl‑2 related protein A1 Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Mouse Bcl‑2 related protein A1 Antibody

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