Mouse BLAME/SLAMF8 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF4156V Datasheet / COA / SDS

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AF4156V-100UG

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All BLAME/SLAMF8 Antibodies

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Mouse BLAME/SLAMF8 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse BLAME/SLAMF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human BLAME is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BLAME/SLAMF8
Val21-Asp231
Accession # Q9D3G2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BLAME/SLAMF8

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of the Ig superfamily cell receptors. The 258 amino acid (aa) mature mouse BLAME contains a 211 aa extracellular domain (ECD) with an Ig V- and a C2-like domain, and a 26 aa cytoplasmic tail that lacks signaling motifs. BLAME is expressed by activated monocytes and dendritic cells. Although the function of BLAME is not known, it is likely involved in homotypic binding or heterotypic interactions with other CD2 subset members. Over the ECD region used as immunogen, mouse BLAME shares 95% and 78% aa identity with rat and human BLAME, respectively.

Long Name

SLAM Family Member 8

Entrez Gene IDs

56833 (Human); 74748 (Mouse); 289237 (Rat); 102122568 (Cynomolgus Monkey)

Alternate Names

B Lymphocyte Activator Macrophage Expressed; BCM-Like Membrane Protein; BLAME; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; CD353; SBBI42; SLAM Family Member 8; SLAMF8