Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CCR5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCR5
Met1-Ala32, Ala92-Lys104, Thr169-Met200, Gln263-Gln279 (Ile11Ser, Ile97Val)
Accession # P51682

Specificity

Detects mouse CCR5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) CCR1, rmCCR4, and rmCCR8 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse CCR5 Antibody

Detection of Mouse CCR5 antibody by Western Blot.

Detection of Mouse CCR5 by Western Blot.

Western blot shows lysates of EL-4 mouse lymphoblast cell line (IL-2 secreting) and mouse thymus tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CCR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6138) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR5 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse CCR5 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: EL‑4 mouse lymphoblast cell line (IL-2 secreting) and mouse thymus tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR5

CCR5 (CC chemokine receptor 5; also CD195 and MIP-1 alpha receptor) is a 41kDa (predicted) member of the G-protein coupled receptor #1 family of proteins. It is expressed on Treg T cells, NK cells, neurons, macrophages and Th1 T cells. CCR5 responds to RANTES, MIP-1 alpha and -1 beta, and induces cell adhesion and migration. Mouse CCR5 is a 7-transmembrane glycoprotein 354 amino acids (aa) in length and it contains an O-glycosylated and sulfated extracellular N-terminus (aa 1-32), plus a phosphorylated and palmitoylated intracellular C-terminus (aa 304-354). In human and primates, CCR5 appears as a 62-64 kDa membrane form, possibly due to extensive posttranslational modification. CCR5 will form homodimers, heterodimers with CCR2, and heterooligomers with CCR2 and CXCR4. Over aa 1-32, 92-104, 169-200 and 263-279 collectively, mouse CCR5 shares 88% and 78% aa identity with rat and human CCR5, respectively.

Alternate Names

CCR5, CD195

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Gene Symbol

CCR5

UniProt

P51682

Additional CCR5 Products

Product Documents for Mouse CCR5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCR5 Antibody

For research use only

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