Mouse CMG-2/ANTXR2 Antibody

R&D Systems | Catalog # AF3636

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CMG-2/ANTXR2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CMG‑2/ANTXR2
Gly26-Gly318
Accession # Q6DFX2

Specificity

Detects mouse CMG‑2/ANTXR2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human CMG-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CMG-2/ANTXR2 Antibody

Detection of Mouse CMG‑2/ANTXR2 antibody by Western Blot.

Detection of Mouse CMG‑2/ANTXR2 by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, mouse thymus tissue, and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse CMG-2/ANTXR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3636) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CMG-2/ANTXR2 at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse CMG-2/ANTXR2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line, mouse thymus tissue, and mouse lung tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CMG-2/ANTXR2

Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1‑4). CMG-2 is a ~55 kDa protein that contains a 31 amino acid (aa) signal sequence, a 287 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 148 aa cytoplasmic domain. Unlike human CMG-2 which has four isoforms, only one sequence has been reported for mouse CMG-2. The main functional domain of CMG‑2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS), through which it adheres selectively to collagen type IV and laminin (1‑6). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (4). In humans, CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (7). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (6, 8, 9). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (10, 11), allows PA pore formation and release of toxin to the cytoplasm (11, 12). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, mouse CMG-2 shares 84%, 91%, 80%, and 83% amino acid sequence homology with human, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.

References

  1. Scobie, H.M. and J.A.T. Young (2005), Curr. Opin. Microbiol. 8:106.
  2. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
  3. Swiss Prot. Accession #: Q6DFX2.
  4. Bell, S.E. et al. (2001) J. Cell Sci. 114:2755.
  5. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367.
  6. Santelli, E. et al. (2004) Nature 430:905.
  7. Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957.
  8. Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349.
  9. Go, M.Y. et al. (2006) J. Mol. Biol. 360:145.
  10. Abrami, L. et al. (2006) J. Cell Biol. 172:309.
  11. Wei, W. et al. (2006) Cell 124:1141.
  12. Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.

Long Name

Capillary Morphogenesis Protein 2

Alternate Names

ANTXR2, cI-35, CMG2, ISH, JHS

Entrez Gene IDs

118429 (Human); 71914 (Mouse)

Gene Symbol

ANTXR2

UniProt

Q6DFX2

Additional CMG-2/ANTXR2 Products

Product Documents for Mouse CMG-2/ANTXR2 Antibody

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Product Specific Notices for Mouse CMG-2/ANTXR2 Antibody

For research use only

Related Research Areas

Infectious Diseases

Citations for Mouse CMG-2/ANTXR2 Antibody

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