Mouse Common gamma Chain/IL‑2 R gamma Antibody

Name: Mouse Common gamma Chain/IL‑2 R gamma Antibody
Brand: R&D Systems
SKU: MAB7843
Price: 399 USD
Availability: InStock

R&D Systems | Catalog # MAB7843

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 644337

View all Common gamma Chain/IL-2 R gamma Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant mouse Common gamma Chain/ IL-2 R gamma
Ser25-Ala263 (predicted)
Accession # P34902

Specificity

Detects mouse Common gamma Chain/ IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Common gamma Chain, recombinant mouse (rm) IL-2 R alpha, or rmIL-2 R beta is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse Common gamma Chain/IL‑2 R gamma Antibody

Detection of Mouse Common gamma Chain/IL‑2 R gamma by Western Blot.

Western blot shows lysates of mouse lymphocytes. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Common gamma Chain/IL-2 R gamma Monoclonal Antibody (Catalog # MAB7843) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Common gamma Chain/IL-2 R gamma at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Common gamma Chain/IL‑2 R gamma Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: Mouse lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Common gamma Chain/IL-2 R gamma

The Common gamma Chain, also known as CD132, is a transmembrane protein belonging to the hematopoietin receptor family. gamma _c is a signaling component of the functional receptor complexes for IL-2, IL-4, IL-7, IL-9, and IL-15.