Mouse Common gamma Chain/IL-2 R gamma Antibody

Name: Mouse Common gamma Chain/IL-2 R gamma Antibody
Brand: R&D Systems
SKU: AF784
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF784

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Common gamma Chain/IL-2 R gamma Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Common gamma Chain/IL‑2 R gamma
Ser25-Ala263
Accession # Q3UPA9

Specificity

Detects mouse Common gamma Chain/IL‑2 R gamma in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) IL-2 R gamma is observed and less than 1% cross-reactivity with rhIL-2 R alpha, recombinant mouse IL-2 R beta, and rhIL-15 R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse Common gamma Chain/IL-2 R gamma Antibody

Cell Proliferation Induced by IL‑15 and Neutralization by Mouse Common gamma Chain/ IL‑2 R gamma Antibody.

Recombinant Human IL-15 (Catalog # 247-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Common gamma Chain/IL-2 R gamma Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF784). The ND₅₀ is typically 6-24 µg/mL.

Applications for Mouse Common gamma Chain/IL-2 R gamma Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Common gamma Chain/IL‑2 R gamma Fc Chimera (Catalog # 784-MR)

Neutralization

Measured by its ability to neutralize IL‑15-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. The Neutralization Dose (ND₅₀) is typically 6-24 µg/mL in the presence of 0.8 ng/mL Recombinant Human IL‑15.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional mouse IL-2 receptor complex belongs to the hematopoietin receptor family. Mouse Common gamma Chain is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 241 residue extracellular domain, a 21 residue transmembrane domain and an 85 residue cytoplasmic domain. Although Common gamma Chain by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma is also designated the common gamma chain ( gamma _c) (1, 2). The site of molecular defects in human X-linked SCID (severe combined immunodeficiency) has been mapped to the human common gamma gene.