Mouse Cystatin B Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB1409U Datasheet / COA / SDS

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FAB1409U-100UG

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All Cystatin B Antibodies

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Mouse Cystatin B Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Cystatin B in direct ELISAs and Western blots. In Western blots, 20‑25% cross-reactivity with recombinant human (rh) Cystatin B is observed and no cross-reactivity with rhCystatin D, S, SA, SN, recombinant mouse (rm) Cystatin

Source

Monoclonal Rat IgG2a Clone # 227818

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse Cystatin B
Met2-Phe98
Accession # Q62426

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cystatin B

Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Cystatin B-deficient mice have increased expression of proteolysis, apoptosis and glial activation genes, which is consistent with the pathology found in the mouse model of human progressive myoclonus epilepsy (EPM1) (3). The mouse Cystatin B consists of 98 amino acid residues (4).

Entrez Gene IDs

1476 (Human); 13014 (Mouse)

Alternate Names

CPI-B; CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B; CSTB; cystatin B (stefin B); Cystatin B; EPM1; Liver thiol proteinase inhibitor; PME; Stefin B; stefin-B