Mouse Cystatin E/M Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB1284T Datasheet / COA / SDS

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FAB1284T-100UG

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All Cystatin E/M Antibodies

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Mouse Cystatin E/M Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Cystatin E/M in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Cystatin B, S, E/M, SA, S/N, recombinant mouse (rm) Cystatin A, C, D, rhFetuin A, B, rmHPRG, or rhKin

Source

Monoclonal Rat IgG2b Clone # 216820

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cystatin E/M
Glu29-Val149
Accession # NP_082899

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cystatin E/M

Cystatin E/M is a member of family 2 of the cystatin superfamily (1 ‑ 6). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice with a null mutation in the cystatin E/M gene have defects in epidermal cornification and die between 5 and 12 days of age (7). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (8). In addition to being a cysteine protease inhibitor, the human cystatin E/M is also a substrate for transglutaminases (9).

Entrez Gene IDs

1474 (Human); 73720 (Mouse)

Alternate Names

CST6; cystatin 6; Cystatin E/M; cystatin M; cystatin M/E; Cystatin-6; cystatin-E; cystatin-M; cysteine proteinase inhibitor