Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry, ELISA Capture, ELISA Development, Immunodepletion

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 355022
View all EBI3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑27 heterodimer
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)

Specificity

Detects mouse IL-27/IL-35 EBI3 Subunit in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human IL‑27/IL-35 EBI3 subunit.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse EBI3 Antibody

Detection of Mouse IL-27/IL-35 EBI3 Subunit antibody by Western Blot.

Detection of Mouse IL‑27/IL‑35 EBI3 Subunit by Western Blot.

Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with mouse EBI3. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit Monoclonal Antibody (Catalog # MAB18341) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for IL-27/IL-35 EBI3 Subunit at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse EBI3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: NS0 mouse myeloma cell line transfected with mouse IL-27/IL-35 EBI3

Reviewed Applications

Read 1 review rated 4 using MAB18341 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EBI3

IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Entrez Gene IDs

10148 (Human); 50498 (Mouse); 680609 (Rat); 102144621 (Cynomolgus Monkey)

Gene Symbol

EBI3

UniProt

O35228 (EBI3) & Q8K3I6 (p28)

Additional EBI3 Products

Product Documents for Mouse EBI3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse EBI3 Antibody

For research use only

Related Research Areas

Regulatory T Cells (Tregs)

Citations for Mouse EBI3 Antibody

Customer Reviews for Mouse EBI3 Antibody (1)

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  • Mouse IL-27/IL-35 EBI3 Subunit Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Culture Supernates
    Species: Mouse
    Verified Customer | Posted 08/06/2018

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