Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 126618
View all Gas6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Gas6
Asp115-Pro674 (Del Pro530)
Accession # Q61592.2

Specificity

Detects mouse Gas6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human Gas6.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Gas6 Antibody

Detection of Gas6 by Western Blot

Detection of Gas6 by Western Blot

MRL MSC produced and released higher amount of MANF than BL6, BALB/c and DBA1 MSC. (A) Proteomic analysis of differential expression of MANF, GAS6, NENF, and SEMA5A in MRL MSC compared to BL6 MSC secretomes. Effect size indicate the standardized mean difference in protein expression level between MRL MSC and BL6 MSC. For each protein, the median intensity levels (Log 2 value) in MRL MSC and BL6 MSC are indicated. Normalized protein intensities were used to calculate the Effect size MRL/BL6. (B) Western blot analysis of MANF, GAS6, NENF, and SEMA5A in whole-cell extracts from BL6, BALB/c and DBA1 MSC. The intensity value of each target protein band was normalized against the intensity value of alpha -Tubulin gel band used as the internal loading control for each sample. (C)MANF mRNA expression level in MRL MSC and BL6 MSC. Western blot analysis of supernatants of BL6, BALB/c, and DBA1 MSC showing blotted proteins stained with Ponceau S (D) and the MANF protein band revealed by the anti-MANF antibody (E). MRL MSC whole cell extract was used as a positive control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33738280), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Gas6 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Gas6 (Catalog # 986-GS)
under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB986 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gas6

Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homolog. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium. Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.

References

  1. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  2. Goruppi, S. et al. (1996) Oncogene 12:471.
  3. Crosier, K and P. Crosier (1997) Pathology 29:131.
  4. Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
  5. Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.

Long Name

Growth-arrest-specific Protein 6

Alternate Names

AXLLG

Entrez Gene IDs

2621 (Human); 14456 (Mouse)

Gene Symbol

GAS6

UniProt

Q61592.2

Additional Gas6 Products

Product Documents for Mouse Gas6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Gas6 Antibody

For research use only

Citations for Mouse Gas6 Antibody

Customer Reviews for Mouse Gas6 Antibody (1)

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  • Mouse Gas6 Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Plasma
    Species: Mouse
    Verified Customer | Posted 11/29/2020
    Mouse Gas6 Antibody MAB986

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