Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all GPVI Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GPVI
Gly24-Lys265
Accession # P0C191

Specificity

Detects mouse GPVI in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GPVI is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse GPVI Antibody

Detection of Mouse GPVI antibody by Western Blot.

Detection of Mouse GPVI by Western Blot.

Western blot shows lysates of mouse platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse GPVI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6758) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GPVI at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Applications for Mouse GPVI Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse platelets

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPVI

GPVI (Platelet Glycoprotein VI; also glycoprotein 5) is a member of the Ig superfamily. It is found on platelets and megakaryocytes, and serves as the main collagen receptor on platelets. Following exposure to subendothelial connective tissue, GPVI binds to a Gly-Pro-(hydroxy)Pro motif on collagen, and generates a noncovalent membrane signaling complex with FcR gamma -chain. This interaction is stabilized by integrin alpha 2 beta 1, followed by activation of PLC gamma 2 with clot initiation. Mature mouse GPVI is a 292 amino acid (aa) type I transmembrane protein. It possesses a 244 aa extracellular region (aa 22-265) that contains two C2-type Ig-like domains (aa 27-197)and two potential glycosylation sites, plus a 37 aa cytoplasmic tail (aa 287-313). There is one potential splice form that shows a deletion of aa 224-240. Over aa 24-265, mouse GPVI shares 70% and 86% aa identity with human and rat GPVI, respectively.

Long Name

Glycoprotein VI [Platelet]

Alternate Names

GP6

Entrez Gene IDs

51206 (Human); 243816 (Mouse)

Gene Symbol

GP6

UniProt

P0C191

Additional GPVI Products

Product Documents for Mouse GPVI Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse GPVI Antibody

For research use only

Related Research Areas

Coagulation

Citations for Mouse GPVI Antibody

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FAQs

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail