Mouse Haptoglobin Alexa Fluor® 405-conjugated Antibody

Catalog #: AF4409V Datasheet / COA / SDS

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AF4409V-100UG

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Mouse Haptoglobin Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Haptoglobin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with human Haptoglobin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse serum-derived Haptoglobin

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Haptoglobin

Haptoglobin is a 45‑48 kDa acute phase glycoprotein that is a naturally inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and protects tissues from hemoglobin-mediated oxidative damage. Mature mouse (pro)haptoglobin is 329 amino acids (aa) in length. Proteolytic cleavage between Arg102-Ile103 creates disulfide-linked alpha - and beta -chains, which subsequently homodimerize through alpha -chain linkages to create a tetramer. Circulating haptoglobin is 70% tetramer and 30% unprocessed (pro)haptoglobin. Mouse (pro)haptoglobin shares 90% and 80% aa identity with rat and human (pro)haptoglobin, respectively.

Entrez Gene IDs

3240 (Human); 15439 (Mouse); 24464 (Rat)

Alternate Names

binding peptide; BP; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; Haptoglobin; haptoglobin, alpha polypeptide; haptoglobin, beta polypeptide; HP; HP-1; hp2-alpha; HP2ALPHA2; HPA1S; MGC111141; zonulin