Mouse HSF4 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF4088X Datasheet / COA / SDS

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AF4088X-100UG

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Mouse HSF4 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse HSF4 in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) HSF4 is observed and less than 5% cross-reactivity with recombinant mouse HSF1 and rhHSF2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse HSF4
Gly343-Pro492
Accession # Q9R0L1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HSF4

HSF4 (heat shock factor 4) is a 55 kDa member of the HSF family of transcription factors. It is highly expressed in lens where it maintains lens integrity and blocks FGF synthesis. Mouse HSF4B is 492 amino acids in length. It contains one DNA binding region (aa 17‑122) and a Leu-zipper trimerization domain (hydrophobic repeat‑A/B) (aa 129‑203). Two additional potential isoforms exist. The first isoform, termed HSF4A, is transcriptionally inactive and shows a 45 aa substitution for aa 245‑319. The second isoform shows an 18 aa substitution for the first 78 amino acids of the N-terminus. Over aa 343‑492, mouse HSF4 shares 93% and 72% aa sequence identity with rat and human HSF4, respectively.

Long Name

Heat Shock Transcription Factor 4

Entrez Gene IDs

3299 (Human); 26386 (Mouse)

Alternate Names

ATHSF4; AT-HSFB1; cataract, Marner; CTM; heat shock factor protein 4; heat shock transcription factor 4CTM; hHSF4; HSF 4; HSF4; HSFB1; HSTF 4