Mouse Iduronate 2-Sulfatase/IDS Alexa Fluor® 532-conjugated Antibody

Catalog #: AF2486X Datasheet / COA / SDS

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AF2486X-100UG

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Mouse Iduronate 2-Sulfatase/IDS Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Iduronate 2‑Sulfatase/IDS in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human IDS is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Iduronate 2â€‘Sulfatase/IDS
Thr36-Pro552
Accession # Q08890

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Iduronate 2-Sulfatase/IDS

As a member of the sulfatase family, Iduronate 2-Sulfatase encoded by the IDS gene is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate (1, 2). It hydrolyzes the 2-sulfate group of the L-iduronate 2-sulfate units of the GAG. The IDS deficiency results in mucopolysaccharidosis II (MPS II or Hunter syndrome), an X-linked inborn error leading to lysosomal accumulation of the GAG and their excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe. The mouse IDS has sulfatase activity. In addition, recombinant human IDS is also available (Catalog # 2449-SU).

Entrez Gene IDs

3423 (Human); 15931 (Mouse)

Alternate Names

Alpha-L-iduronate sulfate sulfatase; EC 3.1.6.13; IDS; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; iduronate 2-sulfatase; idursulfase; MPS2; S; SIDS