Mouse IFN-alpha/beta R1 Antibody

R&D Systems | Catalog # AF3039

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IFN-alpha/beta R1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R1
Glu27-Thr429
Accession # P33896

Specificity

Detects mouse IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) IFN‑ alpha / beta  R2, rmIFN-gamma R1, and rmIFN-gamma R2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IFN-alpha/beta R1 Antibody

Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R1 Antibody.

Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R1 Antibody.

Recombinant Mouse Limitin (Catalog # 1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IFN-a/ beta R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3039). The ND50 is typically 1-4 µg/mL.

Applications for Mouse IFN-alpha/beta R1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IFN-alpha / beta  R1 (Catalog # 3039-AB)

Neutralization

Measured by its ability to neutralize IFN‑ alpha / beta  R1-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.

Reviewed Applications

Read 1 review rated 4 using AF3039 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha/beta R1

IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1-3). IFN-alpha / beta R1, in association with IFN-alpha / beta R2, is required for propagating antiviral signal transduction triggered by IFN-alpha and IFN-beta (4, 5). The mouse IFN-alpha / beta R1 cDNA encodes a 590 amino acid (aa) precursor including a 26 aa signal sequence, a 403 aa extracellular domain (ECD), a 20 aa transmembrane segment, and a 141 aa cytoplasmic domain (6). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. The ECD of mouse IFN-alpha / beta R1 shares 47-48% aa identity with that of human, bovine, porcine, and ovine IFN-alpha / beta R1. IFN-alpha / beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN-alpha / beta R2. Ligands associate with IFN-alpha / beta R2, and this complex subsequently forms a stable ternary assembly with IFN-alpha / beta R1 (7, 8). IFN-alpha / beta R1 also associates with IFN-gamma R2 even in the absence of IFN-gamma stimulation (5). Tyrosine phosphorylation within the juxtamembrane cytoplasmic domain of IFN-alpha / beta R1 provides a docking site for the SH2 domains of Tyk2 and STAT2 (9-11). Tyk2 can directly phosphorylate IFN-alpha / beta R1 (10). Tyk2 also increases the level of cell surface expression of IFN-alpha / beta R1 by preventing constitutive internalization (12). Human IFN-alpha / beta R1 contains a nuclear localization signal in its ECD which is required for receptor translocation to the nucleus following interaction with ligand (13).

References

  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Kotenko, S.V. and J.A. Langer (2004) Int. Immunopharmacol. 4:593.
  3. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  4. Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284.
  5. Takaoka, A. et al. (2000) Science 288:2357.
  6. Uze, G. et al. (1992) Proc. Natl. Acad. Sci. USA 89:4774.
  7. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
  8. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
  9. Krishnan, K. et al. (1998) J. Biol. Chem. 273:19495.
  10. Yan, H. et al. (1996) Mol. Cell. Biol. 16:2074.
  11. Yan, H. et al. (1996) EMBO J. 15:1064.
  12. Ragimbeau, J. et al. (2003) EMBO J. 22:537.
  13. Subramaniam, P.S. and H.M. Johnson (2004) FEBS Lett. 578:207.

Long Name

Interferon alpha/beta Receptor 1

Alternate Names

IFN-aR1, IFN-bR1, IFNAR1, IFNbR1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)

Gene Symbol

IFNAR1

UniProt

P33896

Additional IFN-alpha/beta R1 Products

Product Documents for Mouse IFN-alpha/beta R1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse IFN-alpha/beta R1 Antibody

For research use only

Citations for Mouse IFN-alpha/beta R1 Antibody

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  • Mouse IFN-alpha / beta  R1 Antibody
    Name: zhao zhan
    Application: Block/Neutralize
    Sample Tested: Bone Marrrow Derived Macrophages
    Species: Mouse
    Verified Customer | Posted 11/22/2019
    Different concentration of anti-IFN alpha / beta R1 block M-CSF induced BMDM for 30 min, then stimulated by 100 ng/ml LPS for 4 h
    Mouse IFN-alpha/beta R1 Antibody AF3039

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Associated Pathways

Jak/STAT Signaling Pathway
Jak/STAT Signaling Pathway Thumbnail