Mouse IFN-beta Antibody

Catalog #: MAB8234
1 Citation Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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MAB8234-100
MAB8234-SP

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IFN‑ beta Inhibition of Viral-induced Cytopathy and Neutralization by Mouse IFN‑ beta Antibody.

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All IFN-beta Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFN-beta Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IFN‑ beta in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1176D

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived mouse IFN‑ beta
Ile22-Asn182
Accession # P01575

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize IFN‑ beta inhibition of viral-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.25-1.25 µg/mL in the presence of 10 pg/mL Recombinant Mouse IFN‑ beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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IFN‑ beta Inhibition of Viral-induced Cytopathy and Neutralization by Mouse IFN‑ beta Antibody. Recombinant Mouse IFN-beta (Catalog # 8234-MB) reduces the viral-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Inhibition of viral activity elicited by Recombinant Mouse IFN-beta (10 pg/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse IFN-beta Monoclonal Antibody (Catalog # MAB8234). The ND₅₀ is typically 0.25-1.25 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).

References

González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
Häcker, H. et al. (2006) Nature 439:204.
Teige, I. et al. (2003) J. Immunol. 170:4776.
Shinohara, M.L. et al. (2008) Immunity 29:68.
Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
Wang, H. et al. (2011) J. Immunol 186:675.

Long Name

Interferon beta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Alternate Names

Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse IFN-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity
Authors: Zhang, H;Xue, K;Li, W;Yang, X;Gou, Y;Su, X;Qian, F;Sun, L;
Nature communications
Species: Mouse, Transgenic Mouse
Sample Types: In Vivo
Applications: In vivo assay