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Mouse IGFALS/ALS Antibody

Catalog #: MAB1436
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1436
MAB1436-SP

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Detection of Mouse IGFALS/ALS by Western Blot.
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Mouse IGFALS/ALS Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGFALS/ALS in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG1 Clone # 199410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFALS/ALS
Thr28-Cys603
Accession # P70389
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IGFALS/ALS antibody by Western Blot. View Larger

Detection of Mouse IGFALS/ALS by Western Blot. Western blot shows mouse serum. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse IGFALS/ALS Monoclonal Antibody (Catalog # MAB1436) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for the ALS/IGFBP-3 Complex at approximately 140 kDa (as indicated) and ALS at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFALS/ALS

Acid Labile Subunit (ALS) is a liver-derived serum protein that forms a ternary complex with IGFBP-3 and either IGF-I or IGF-II. The presence of ALS increases the half life of IGF/IGFBP-3 complexes in the circulation.

Long Name
IGF-binding Protein Acid Labile Subunit
Entrez Gene IDs
3483 (Human); 16005 (Mouse)
Alternate Names
ACLSD; ALS; IGFALS

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Citation for Mouse IGFALS/ALS Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The ternary IGF complex influences postnatal bone acquisition and the skeletal response to intermittent parathyroid hormone.
    Authors: Yakar S, Bouxsein ML, Canalis E, Sun H, Glatt V, Gundberg C, Cohen P, Hwang D, Boisclair Y, LeRoith D, Rosen CJ
    J. Endocrinol., 2006-05-01;189(2):289-99.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

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