Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IGFALS/ALS Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IGFALS/ALS
Thr28-Cys603
Accession # P70389

Specificity

Detects mouse IGFALS/ALS in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IGFALS/ALS Antibody

Detection of Mouse IGFALS/ALS antibody by Western Blot.

Detection of Mouse IGFALS/ALS by Western Blot.

Western blot shows lysates of mouse spleen tissue, mouse liver tissue, and mouse bone marrow. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse IGFALS/ALS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1436) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFALS/ALS at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IGFALS/ALS Antibody

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: Mouse spleen tissue, mouse liver tissue, and mouse bone marrow

Reviewed Applications

Read 1 review rated 5 using AF1436 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGFALS/ALS

ALS (Acid labile subunit) is an 84-86 kDa glycoprotein member of the leucine rich repeat superfamily of molecules.  It is secreted by hepatocytes in response to growth hormone stimulation, and forms a 140-150 kDa ternary complex with IGFBP3 (or IGFBP5) and IGF-I.  This complex increases the half-life of IGF-I and retains it in the circulation for use in multiple tissues.  Mature mouse ALS is 580 amino acids (aa) in length (aa 24-603).  It contains twenty-one 20-23 aa LRRs that participate in protein-protein interactions.  There are two potential isoform variants that show N-terminal extensions.  One contains a 66 aa substitution for aa 1-5, while another possesses an 89 aa substitution again for aa 1-5.  Over aa 28-603, mouse ALS shares 79% and 93% aa identity with human and rat ALS, respectively.        

Long Name

IGF-binding Protein Acid Labile Subunit

Alternate Names

ACLSD, ALS

Entrez Gene IDs

3483 (Human); 16005 (Mouse)

Gene Symbol

IGFALS

UniProt

P70389

Additional IGFALS/ALS Products

Product Documents for Mouse IGFALS/ALS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IGFALS/ALS Antibody

For research use only

Citations for Mouse IGFALS/ALS Antibody

Customer Reviews for Mouse IGFALS/ALS Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22999939
    Species: Mouse
    Verified Customer | Posted 01/05/2015

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