Mouse IgG2A Isotype Control

R&D Systems | Catalog # MAB0031

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Key Product Details

Species Reactivity

Mouse

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 133304
View all IgG2A Isotype Controls »

Product Specifications

Immunogen

KLH

Specificity

Recognizes KLH (Keyhole Limpet Hemocyanin).  In immunohistochemistry or flow cytometry of a variety of cell types or tissues, no background staining is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IgG2A Isotype Control

Detection of Mouse IgG Isotype Control by Flow Cytometry

Detection of Mouse IgG Control by Flow Cytometry

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 Monoclonal Antibody (Catalog # MAB1298, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB0031, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog F0101B).

Applications for Mouse IgG2A Isotype Control

Application
Recommended Usage

Control

Negative control for flow cytometry, immunohistochemistry, or immunocytochemistry experiments. Use at the same concentration as the detection antibody.

Reviewed Applications

Read 2 reviews rated 5 using MAB0031 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2A

IgG is the most abundant of the five classes of mammalian immunoglobulins in serum. IgG molecules (~150 kDa) consist of two ~50 kDa heavy chains and two 25 kDa light chains connected by disulfide bonds. Antibody specificity is conferred by a combination of somatic rearrangement and hypermutation within variable regions. Mouse IgG isotypes IgG1, IgG2a and IgG2b share approximately 64-78% amino acid sequence identity within the heavy chain constant region domains (CH1, CH2 and CH3) and may vary in their interaction with complement, NK and mast cells, induction or inhibition by specific cytokines, and ability to cross the placenta.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Mouse IgG2A Isotype Control

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IgG2A Isotype Control

For research use only

Citations for Mouse IgG2A Isotype Control

Customer Reviews for Mouse IgG2A Isotype Control (2)

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  • Mouse IgG2A Isotype Control
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: ?TC-6 mouse beta cell insulinoma cell line
    Species: Human and Mouse
    Verified Customer | Posted 08/17/2016
    PFA fixation. Used between 5ug/mL and 0.5ug/mL. No signals until respectively 500 and 750msec of acquisition.
  • Mouse IgG2A Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human Placenta
    Species: Mouse and Human
    Verified Customer | Posted 02/22/2016

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