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Mouse IgG2B Isotype Control (20116)

R&D Systems | Catalog # MAB004

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Key Product Details

Species Reactivity

Mouse

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 20116
View all IgG2B Isotype Controls »

Product Specifications

Immunogen

KLH

Specificity

Selected for use as mouse IgG2B isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta ), rhBLC/BCA-1, rmBLC, rhBRAK, rmBRAK, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC‑2 beta, rrCINC-3, rhCK beta 8-1, rmCRG-2, rmCTACK, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmI-TAC, rmJE, rmKC, rhLeukotactin-1, rrLIX, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rhMIP-1 delta, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, recombinant viral (rv) MIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF‑1 alpha, rmSDF‑1 alpha, rhSDF‑1 beta, rhTarc, rmTarc, rmTCA-3, rhTeck, rmTeck, rhVIC, or rmVIC.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Mouse IgG2B Isotype Control (20116)

Detection of Mouse IgG Isotype Control by Flow Cytometry

Detection of Mouse IgG Control by Flow Cytometry

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog # MAB89001, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog F0101B).
Detection of IgG2B by Flow Cytometry

Detection of IgG2B by Flow Cytometry

Prolonged stimulation through activating receptors induces NK cell exhaustion.(A) Schematic representing the in vitro model of exhaustion. Agonists of NKp46 (anti-NKp46) and NKG2D (MICA and MICB) were adsorbed onto tissue culture plates and used to stimulate NK cells for 7 days. Plate-bound isotype IgG served as a control. Both groups received 1 ng/mL IL-15. (B) NK cells harvested from isotype-coated and exhaustion plates (day 7) were incubated with K-562 targets for 4 hours (E/T: 2:1). Cytokine production (IFN-gamma and TNF-alpha ) and degranulation (CD107a) were measured via flow cytometry. Isotype NK cells (top row) in blue, exhausted NK cells (bottom row) in red. E/T, effector/target. (C–E) Quantification of cytokine production and degranulation as percentage of parent population (live, CD3–CD56+ cells) and mean fluorescence intensity (MFI) (n = 4). Paired t tests were used for comparisons. **P < 0.01; ***P < 0.001; ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://insight.jci.org/articles/view/150079), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IgG2B by Flow Cytometry

Detection of IgG2B by Flow Cytometry

Prolonged stimulation through activating receptors results in functional and proliferative defects in vivo.(A) Schematic representing experimental design: sublethally irradiated NSG mice were injected (i.v.) with HL-60-Luc2 cells and injected (i.v.) with NK cells 3 days later. NK cells had been incubated for 7 days on plates with either isotype IgG (control) or anti-NKp46 and MICA/B as previously described. (B) Fourteen days after NK cell injection, blood was drawn, and NK cells were counted via flow cytometry. NK cells were human CD45+CD56+CD3–. One-way ANOVA was used for comparisons (n = 8). *P ≤ 0.05. (C) HL-60 tumor burden was tracked via bioluminescent imaging (BLI), with day 21 data pictured. One-way ANOVA was used for comparisons (n = 8). ***P < 0.001. (D and E) Fourteen days after NK cell injection, NK cells were restimulated with K-562 leukemia cells for 4 hours as previously described. Ki67, IFN-gamma, TNF-alpha, and CD107a expression was assessed via flow cytometry (D). Paired t tests were used for comparisons (n = 8). *P ≤ 0.05. Representative data depict Ki67 and IFN-gamma expression following K-562 restimulation (E). Image collected and cropped by CiteAb from the following open publication (https://insight.jci.org/articles/view/150079), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IgG2B by Flow Cytometry

Detection of IgG2B by Flow Cytometry

Prolonged stimulation through activating receptors alters expression of activating and inhibitory receptors.(A–H) NK cells were harvested from isotype-coated and exhaustion plates (day 7), and expression of TIGIT (A and B) (n = 5), CD96 (C and D) (n = 3, n = 5), CD16 (E and F) (n = 5), and NKG2D (G and H) (n = 6) was measured via flow cytometry. (I) At day 7 of stimulation, cells from 3 donors were analyzed via mass cytometry (CyTOF). Heatmap indicates fold change relative to isotype mean metal intensity. Markers are ordered from most downregulated to most upregulated relative to control. Paired t tests were used for comparisons (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://insight.jci.org/articles/view/150079), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IgG2B Isotype Control (20116)

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.

Reviewed Applications

Read 8 reviews rated 4.6 using MAB004 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Mouse IgG2B Isotype Control (20116)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IgG2B Isotype Control (20116)

For research use only

Citations for Mouse IgG2B Isotype Control (20116)

Customer Reviews for Mouse IgG2B Isotype Control (20116) (8)

4.6 out of 5
8 Customer Ratings
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Customer Images

Showing  1 - 5 的 8 reviews Showing All
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  • Mouse IgG2B Isotype Control
    Name: Lu Bai
    Application: Flow Cytometry
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 10/11/2022
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 08/18/2021
    Mouse IgG2B Isotype Control (20116) MAB004
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Myotubes
    Species: horse
    Verified Customer | Posted 12/04/2020
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MDAMB231
    Species: Human
    Verified Customer | Posted 11/16/2018
    Mouse IgG2B Isotype Control (20116) MAB004
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Verified Customer | Posted 09/24/2018
    Mouse IgG2B Isotype Control (20116) MAB004
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human cell line
    Species: Human
    Verified Customer | Posted 03/15/2018
    Mouse IgG2B Isotype Control (20116) MAB004
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Verified Customer | Posted 03/09/2018
    Mouse IgG2B Isotype Control (20116) MAB004
  • Mouse IgG2B Isotype Control
    Name: Ahmad Al-Attar
    Application: Flow Cytometry
    Sample Tested: Blood-derived lymphocytes
    Species: Human
    Verified Customer | Posted 05/02/2016
    The flow cytometry histogram shows the Mouse IgG2B Isotype Control (red) and TLR-2 (blue). The lymphocytes were gated on based on their FSC/SSC characteristics.
    Mouse IgG2B Isotype Control (20116) MAB004

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