Mouse IkB-epsilon Antibody

Name: Mouse IkB-epsilon Antibody
Brand: R&D Systems
SKU: AF4637
Price: 149 USD
Availability: InStock

R&D Systems | Catalog # AF4637

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
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Specific Notices
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

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Product Specifications

Immunogen

E. coli-derived recombinant mouse I kappa B-epsilon
Met1-Asp364
Accession # O54910

Specificity

Detects endogenous mouse IkB-epsilon in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse IkB-epsilon Antibody

Detection of Mouse IkB-epsilon by Western Blot.

Western blot shows lysates of DA3 mouse myeloma cell line. PVDF membrane was probed with 1 µg/mL of Mouse IkB-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4637) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IkB-e at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse IkB-epsilon Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: DA3 mouse myeloma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IkB-epsilon

NF kappa B inhibitor epsilon (IkB-epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.