Mouse IKK beta Antibody

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Detection of Mouse IKK beta  by Western Blot.
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Product Details
Citations (4)
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Mouse IKK beta Antibody Summary

Species Reactivity
Detects mouse IKK beta in direct ELISAs and Western blots.
Monoclonal Rat IgG2A Clone # 725818
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse IKK beta
Accession # O88351
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IKK beta  antibody by Western Blot. View Larger

Detection of Mouse IKK beta by Western Blot. Western blot shows lysates of BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse IKK beta Monoclonal Antibody (Catalog # MAB7155) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for IKK beta at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK beta

I kappa B kinase beta (IKK beta ) is also known as IKBKB and IKK2. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF kappa B. Like IKK alpha, IKK beta contains kinase (aa 15-300), leucine zipper (aa 458‑479), and helix-loop-helix (aa 605-644) domains. NF kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha /IKK beta heterodimers. Within amino acids 530‑757, mouse IKKb shares 89% and 96% aa sequence identity with human and rat IKKb, respectively.

Long Name
IkB Kinase beta
Entrez Gene IDs
3551 (Human); 16150 (Mouse); 84351 (Rat)
Alternate Names
EC 2.7.11; EC; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK2; IKK2FLJ38368; IKK-B; IKK-betaFLJ36218; IKKBFLJ40509; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; NFKBIKBFLJ33771; Nuclear factor NF-kappa-B inhibitor kinase beta

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Citations for Mouse IKK beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. IL-3-Induced Immediate Expression of c-fos and c-jun Is Modulated by the IKK2-JNK Axis
    Authors: H Fujita, T Fujita, H Fujii
    Cells, 2022-04-25;11(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Uncaria tomentosa improves insulin sensitivity and inflammation in experimental NAFLD
    Authors: LCC Araujo, KB Feitosa, GM Murata, IC Furigo, SA Teixeira, CF Lucena, LM Ribeiro, MN Muscará, SKP Costa, J Donato, S Bordin, R Curi, CRO Carvalho
    Sci Rep, 2018-07-20;8(1):11013.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. IL6 mediated inflammatory loop reprograms normal to EMT(+) metastatic CSCs in pre-neoplastic liver of TGF? deficient ?2SP(+/-) mice
    Authors: Abhisek Mitra
    Hepatology, 2017-01-20;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.
    Authors: Wertz I, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman R, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother W, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee W, Goodnow C, Balazs M, Yu K, Kolumam G, Dixit V
    Nature, 2015-12-09;528(7582):370-5.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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