Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively.
Mouse IL-12 p70 Antibody (48110)
R&D Systems | Catalog # MAB419
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Neutralization, Immunocytochemistry, ELISA Development, ELISA Development (Capture), Functional Assay, Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 48110
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12
Specificity
Detects mouse IL-12 in ELISAs and Western blots. Detects an epitope in the p35 subunit. In sandwich ELISAs, does not cross-react with recombinant mouse IL-12 p40.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Applications for Mouse IL-12 p70 Antibody (48110)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse IL‑12 (Catalog # 419-ML)
under non-reducing conditions only
Sample: Recombinant Mouse IL‑12 (Catalog # 419-ML)
under non-reducing conditions only
Mouse IL-12 p70 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB419 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-12 p70
Long Name
Interleukin 12 p70
Alternate Names
IL12 p70
Gene Symbol
IL12A
Additional IL-12 p70 Products
Product Documents for Mouse IL-12 p70 Antibody (48110)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IL-12 p70 Antibody (48110)
For research use only
Citations for Mouse IL-12 p70 Antibody (48110)
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Customer Reviews for Mouse IL-12 p70 Antibody (48110) (1)
5 out of 5
1 Customer Rating
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Application: ELISASample Tested: Serum and PlasmaSpecies: MouseVerified Customer | Posted 11/27/2017This antibody was paired with BAF419 to create an ELISA to measure IL-12 in mouse samples.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
