Mouse IL-27 p28/IL-30 Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # FAB7430AFP594

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 744015
View all IL-27 p28/IL-30 Primary Antibodies »

Product Specifications

Specificity

Detects mouse IL-27 p28/IL-30 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-27/IL-35 EBI3 subunit, recombinant human (rh) IL-27/IL-35 EBI3 subunit, or rhIL-27 heterodimer is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse IL-27 p28/IL-30 Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IL-27 p28/IL-30

Interleukin-27 p28, also known as IL-30, is a secreted 28 kDa protein that is considered a member of the IL-6/IL-12 interfamily cytokine family. p28/IL-30 is one of several alpha -chain proteins that can associate with various beta -chain proteins to form heterodimeric cytokines in these families. The alpha -chains (e.g. IL-6, IL-11, Cardiotrophin-1, CLC/CNTF, LIF, Oncostatin M, IL-23 p19, IL-27 p28/IL-30, IL-12/IL-35 p35) have a four-helix bundle structure, while the beta -chains (e.g. EBI-3, CLF-1, IL-12/IL-23 p40) resemble class 1 cytokine receptors. These cytokines utilize heteromeric cell surface receptors which contain shared as well as ligand-specific subunits. Divergent biological responses are obtained from the combinatorial association of cytokine subunits and their interaction with various combinations of receptor subunits. Complexity in this system is increased by the generation of soluble receptors and by the competition between proteins for shared subunit pairing (1-3). p28/IL-30 is expressed by macrophages and dendritic cells, and is upregulated in these cells by inflammatory stimuli (4-9). It was first described as a partner with EBI-3 in the heterodimeric cytokine IL-27 (4). IL-27 signals through a receptor complex composed of IL-27 R alpha /WSX-1/TCCR and gp130 (4, 10, 11). This interaction enhances the proinflammatory activation of naïve CD4+ T cells, NK cells, mast cells, and monocytes and the cytotoxic activity of CD8+ T cells (4, 10, 12). IL-27 also exhibits anti-inflammatory activity, including the induction of IL-10 production by naïve and memory T cells, the activation of regulatory T cells (Treg), and the suppression of Th17 cytokine secretion (13, 14). Alternatively, p28/IL-30 associates with CLF-1 to create a cytokine that triggers responses through IL‑27 R alpha, IL‑6 R alpha, and gp130 (6). Like IL-27, p28-CLF-1 heterodimers costimulate IFN-gamma production by NK cells, and induce IL-10 secretion by CD4+ T cells (6). In contrast to IL-27, however, p28-CLF-1 is reported to promote the differentiation of Th17 cells (6). A third mode of p28 action enables it to stimulate cells that express both IL‑6 R alpha and gp130, but lack IL-27 R alpha (11). Similar to the IL-6 system, the presence of IL-6 R alpha on the cell surface is not even required if p28/IL-30 associates with a soluble form of IL-6 R alpha. This combination can trigger trans signaling through gp130, a mechanism that has been demonstrated for complexes of IL-6 with soluble IL‑6 R alpha (11). Overexpression of p28/IL-30 in vivo interferes with humoral antibody responses and protects from IL-12 induced liver inflammation (7, 15). Mature mouse p28/IL-30 shares 70% and 89% amino acid sequence identity with human and rat p28, respectively.

References

  1. Hunter, C.A. and R. Kastelein (2012) Immunity 37:960.
  2. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  3. Vignali, D.A.A. and V.K. Kuchroo (2012) Nat. Immunol. 13:722.
  4. Pflanz, S. et al. (2002) Immunity 16:779.
  5. Liu, J. et al. (2007) J. Exp. Med. 204:141.
  6. Crabé, S. et al. (2009) J. Immunol. 183:7692.
  7. Stumhofer, J.S. et al. (2010) Nat. Immunol. 11:1119.
  8. Bosmann, M. et al. (2012) J. Immunol. 188:5086.
  9. Dibra, D. et al. (2012) J. Immunol. 188:3709.
  10. Pflanz, S. et al. (2004) J. Immunol. 172:2225.
  11. Garbers, C. et al. (2013) J. Biol. Chem. 288:4346.
  12. Schneider, R. et al. (2011) Eur. J. Immunol. 41:47.
  13. Diveu, C. et al. (2009) J. Immunol. 182:5748.
  14. Hall, A.O, et al. (2012) Immunity 37:511.
  15. Dibra, D. et al. (2012) Hepatology 55:1204.

Long Name

Interleukin 27 p28

Alternate Names

IL-30, IL27 p28

Entrez Gene IDs

246778 (Human); 246779 (Mouse)

Gene Symbol

IL27

UniProt

Q8K3I6

Additional IL-27 p28/IL-30 Products

Product Documents for Mouse IL-27 p28/IL-30 Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse IL-27 p28/IL-30 Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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