Mouse Mast Cell Protease-1/Mcpt1 Antibody

Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Western Blot.

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Mast Cell Protease-1/Mcpt1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5146) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Simple Western^TM.

Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 34 kDa (as indicated) using 50 µg/mL of Sheep Anti-Mouse Mast Cell Protease-1/Mcpt1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5146) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mast Cell Protease-1/Mcpt1 by Immunohistochemistry

Mrgprb2 expression in the mouse colon.A, Bar chart showing Mrgprb2 mRNA expression as quantified by qPCR in the skin (left gray bar), in dorsal root ganglia (right gray bar), and colon (red bar). Gene expression is normalized to the reference genes hprt1 and rps29 and relative to the control group (skin). n.d = not detected. B, Representative images of RNAscope in situ hybridization for Mrgprb2 mRNA expression on the skin (left) and colon (right) of healthy mice. Avidin-FITC (green) counterstaining was used as a marker for CTMCs. C, Schematic representation of the transgenic Mrgprb2-cretdTomato mouse design. D, Representative image showing Mrgprb2 co-expression in CD117+ colonic MCs (white arrowheads). E, Mrgprb2+ MCs (white arrowheads) do not co-express Mcpt1, a common mucosal MC marker (open arrowheads). Mrgprb2+ MCs co-express Mcpt6, a connective tissue MC marker (white arrowheads). F, Schematic Venn diagram indicating that Mrgprb2 is selectively expressed in the subset of colonic CTMCs. Cell numbers are pooled of 3 animals. Scale bars represent 50 μm. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2352345X24001462), licensed under a CC-BY license. Not internally tested by R&D Systems.