Mouse MGMT Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB3299T Datasheet / COA / SDS

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FAB3299T-100UG

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Mouse MGMT Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MGMT in Western blots.

Source

Monoclonal Rat IgG2a Clone # 300008

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse MGMT
Met1-Asn211
Accession # P26187

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MGMT

The O-6-methylguanine-DNA-methyl transferase (MGMT) is involved in the direct repair of DNA alkylated at the O⁶ position of guanine. MGMT transfers the alkyl group to its own cysteine thereby inactivating the MGMT molecule. Therefore, the number of MGMT molecules correlates directly with the cell’s ability to withstand DNA alkylation.

Long Name

O-6 Methylguanine-DNA Methyltransferase

Entrez Gene IDs

4255 (Human); 17314 (Mouse)

Alternate Names

6-O-methylguanine-DNA methyltransferase; EC 2.1.1.63; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase