Mouse Neutrophil Elastase/ELA2 Antibody (887105)

R&D Systems | Catalog # MAB4517

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 887105
View all Neutrophil Elastase/ELA2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Neutrophil Elastase/ELA2
Met1-Arg260
Accession # Q3UP87

Specificity

Detects mouse Neutrophil Elastase/ELA2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Neutrophil Elastase/ELA2 Antibody (887105)

Detection of Mouse Neutrophil Elastase/ELA2 antibody by Western Blot.

Detection of Mouse Neutrophil Elastase/ELA2 by Western Blot.

Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB4517) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Neutrophil Elastase/ELA2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Neutrophil Elastase/ELA2 Antibody (887105)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Mouse bone marrow

Reviewed Applications

Read 1 review rated 4 using MAB4517 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as Leukocyte Elastase, Bone marrow serine protease, Medullasin and ELANE, is a 25-30 kDa serine protease belonging to the peptidase S1 family. ELA2 is secreted by neutrophils during inflammation, it destroys bacteria and host tissue. ELA2 function is the degradation of many extracellular matrix macromolecules. ELA2 is localized to keratohyalin granules, where it was found to directly participate in pro-filaggrin processing.  When expressed aberrantly, ELA2 can cause emphysema or emphysematous changes, it involves breakdown of the lung structure and increased airspaces. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity.

Alternate Names

ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin

Entrez Gene IDs

1991 (Human); 50701 (Mouse)

Gene Symbol

ELANE

UniProt

Q3UP87

Additional Neutrophil Elastase/ELA2 Products

Product Documents for Mouse Neutrophil Elastase/ELA2 Antibody (887105)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Neutrophil Elastase/ELA2 Antibody (887105)

For research use only

Citations for Mouse Neutrophil Elastase/ELA2 Antibody (887105)

Customer Reviews for Mouse Neutrophil Elastase/ELA2 Antibody (887105) (1)

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  • Mouse Neutrophil Elastase/ELA2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 07/21/2017
    - loaded 10, 20, 30ug of mouse kidney tissue - blocked and diluted ELA2 Ab in 5% milk (1:1000) - not sure what the HMW bands is though
    Mouse Neutrophil Elastase/ELA2 Antibody (887105) MAB4517

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