Mouse OSCAR Antibody (218311)

Detection of OSCAR by Western Blot

Vitamins D and K inhibited osteoclastogenesis via joint inhibition of OSCAR.A. Venn blot showing the screening strategy of differentially expressed genes.B. Heat map analysis of differentially expressed genes in panel A.C. KEGG enrichment analysis of differentially expressed genes in panel A.D-E. WB analysis of OSCAR (n = 3).E. WB analysis of osteoclast marker proteins NFATC1, CTSK, and C-FOS after OSCAR knockdown.F. TRAP staining of osteoclasts after OSCAR knockdown. Scale bar, 50 μmG. Quantitative analysis of osteoclast number assessed in panel F (n = 3).H. Pit assay showing bone resorption pits by osteoclasts after OSCAR knockdown. Scale bar, 50 μm.I. Proportion of osteolysis area assessed in panel H (n = 3).J-K. WB analysis of OSCAR, NFATC1, CTSK, and C-FOS after OSCAR overexpression and treatment with vitamins D and K.L. TRAP staining of osteoclasts after OSCAR overexpression and treatment with vitamins D and K. Scale bar, 50 μmM. Quantitative analysis of osteoclasts number assessed in panel L (n = 3).N. Pit assay showing bone resorption pits by osteoclasts after OSCAR overexpression and treatment with vitamins D and K. Scale bar, 50 μm.O. Proportion of osteolysis area assessed in panel N (n = 3).ns. None significant. ∗, P value < 0.05. ∗∗, P value < 0.01. ∗∗∗, P value < 0.001. ∗∗∗∗, P value < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2214031X25000543), licensed under a CC-BY license. Not internally tested by R&D Systems.