Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PACAP/ADCYAP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse PACAP/ADCYAP1
Pro26-Leu175
Accession # O70176

Specificity

Detects mouse PACAP/ADCYAP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 12% cross‑reactivity with recombinant human PACAP is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse PACAP/ADCYAP1 Antibody

Detection of Mouse PACAP/ADCYAP1 antibody by Western Blot.

Detection of Mouse PACAP/ADCYAP1 by Western Blot.

Western blot shows lysates of mouse kidney tissue. PVDF Membrane was probed with 1 µg/mL of Human PACAP/ADCYAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PACAP/ADCYAP1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2

Applications for Mouse PACAP/ADCYAP1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PACAP/ADCYAP1

PACAP (Pituitary adenylate cyclase activating polypeptide) is a 17 kDa (predicted) member of the VIP/secretin/glucagon/GHRH superfamily of peptides. It is expressed by anterior pituitary gonadotrophs, neurons in the CNS and PNS, spermatids, adrenal chromaffin cells, plus macrophages and lymphocytes. PACAP has multiple effects. On mast cells, it induces histamine release; on lymphocytes, PACAP promotes Th2 development; in bone, PACAP inhibits osteoclastogenesis; and in the pituitary, PACAP facilitates the release of LH (in females), GH and alpha ‑MSH. Mouse PACAP proprecursor is 151 amino acids (aa) in length (aa 25‑175). It undergoes proteolytic processing to generate two C‑terminal amidated peptides termed PACAP-38 (aa 131‑168) and PACAP-27 (aa 131‑157). In general, PACAP-38 represents the dominant form. However, the ratio between the -38 and -27 forms differs in a cell-specific manner. Over aa 25‑175, mouse PACAP shares 95% and 81% aa identity with rat and human PACAP, respectively.

Long Name

Pituitary Adenylate Cyclase-activating Polypeptide

Alternate Names

ADCYAP1

Entrez Gene IDs

116 (Human); 11516 (Mouse); 24166 (Rat)

Gene Symbol

ADCYAP1

UniProt

O70176 (Mouse)

Additional PACAP/ADCYAP1 Products

Product Documents for Mouse PACAP/ADCYAP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse PACAP/ADCYAP1 Antibody

For research use only

Citations for Mouse PACAP/ADCYAP1 Antibody

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