Mouse PD-L1/B7-H1 Antibody (179711)

R&D Systems | Catalog # MAB1019

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 179711
View all PD-L1/B7-H1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PD-L1/B7-H1
Phe19-Thr238
Accession # Q9EP73

Specificity

Detects mouse PD-L1/B7-H1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) B7-1, rmB7-2, recombinant human (rh) B7-H1, rmB7-H2, rmB7-H3, rmB7-H4, or rmPD-L2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse PD-L1/B7-H1 Antibody (179711)

Detection of PD-L1/B7-H1 by Flow Cytometry

Detection of PD-L1/B7-H1 by Flow Cytometry

PD-L1 is critical for mammary regeneration in transplantation. (A) Illustration of the 3D culture and transplantation assays. Mammary basal cells were isolated by FACS from WT, PD-L1+/– or PD-L1–/– mice for 3D Matrigel culture or transplantation experiments. (B) Colony size of basal cells from WT, PD-L1+/–, or PD-L1–/– mice in Matrigel culture. Data are presented as mean ± SD. Scale bars, 50 μm. (C) Regeneration frequency of WT, PD-L1+/–, or PD-L1–/– basal cells. The mammary outgrowth numbers and sizes (shown as the percentage of fat pad filled) are combined from three independent experiments. The mammary reconstitution unit (MRU) frequency is shown for each group. Student’s t-test: **** P < 0.0001. (D) Representative whole-mount images of outgrowths from transplantation of WT, PD-L1+/–, or PD-L1–/– basal cells. Scale bars, 1 mm. (E) Regeneration efficiency of pWPI or pWPI-PD-L1 primary mammary cells. The mammary outgrowth numbers and sizes (shown as the percentage of fat pad filled) are combined from three independent experiments. The mammary reconstitution unit (MRU) frequency is shown for each group. Student’s t-test: **** P < 0.0001. (F) Representative whole-mount images of outgrowths from transplantation of pWPI or pWPI-PD-L1 primary mammary cells. Scale bars, 0.5 mm. FACS, fluorescence-activated cell sorting; PD-L1, programmed cell death ligand 1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34805179), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse PD-L1/B7-H1 Antibody (179711)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse PD-L1/B7-H1 Fc Chimera (Catalog # 1019-B7)

Reviewed Applications

Read 5 reviews rated 4 using MAB1019 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L1/B7-H1

Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium
(1‑4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-gamma treatment induces B7-H1/PD-L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also beenreported (1-4).

References

  1. Tamura, H. et al. (2001) Blood 97:1809.
  2. Freeman, G. et al. (2000) J. Exp. Med. 192:1027.
  3. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  4. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Q9EP73

Additional PD-L1/B7-H1 Products

Product Documents for Mouse PD-L1/B7-H1 Antibody (179711)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse PD-L1/B7-H1 Antibody (179711)

For research use only

Citations for Mouse PD-L1/B7-H1 Antibody (179711)

Customer Reviews for Mouse PD-L1/B7-H1 Antibody (179711) (5)

4 out of 5
5 Customer Ratings
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4 Stars
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1 Stars
20%

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Customer Images

Showing  1 - 5 的 5 reviews Showing All
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  • Mouse PD-L1/B7-H1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 4T1 cells whole cell lysate and CT28
    Species: Mouse
    Verified Customer | Posted 04/03/2020
    Mouse PD-L1/B7-H1 Antibody (179711) MAB1019
  • Mouse PD-L1/B7-H1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: CT26 and 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 10/28/2018
    Mouse PD-L1/B7-H1 Antibody (179711) MAB1019
  • Mouse PD-L1/B7-H1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Breast cancer cells
    Species: Mouse
    Verified Customer | Posted 07/11/2018
    Mouse PD-L1/B7-H1 Antibody (179711) MAB1019
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Mouse PD-L1/B7-H1 Antibody
    Name: Leslie Priddy
    Application: Cell depletion
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 04/04/2018
  • Mouse B7‑H1/PD‑L1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse splenocytes
    Species: Mouse
    Verified Customer | Posted 10/26/2015
    Buffer: tbst<br />Dilution: 1/1000

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