Mouse Peptidase Inhibitor 16/PI16 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # AF4929AFP680

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Polyclonal Goat IgG
View all Peptidase Inhibitor 16/PI16 Primary Antibodies »

Product Specifications

Specificity

Detects mouse Peptidase Inhibitor 16/PI16 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Peptidase Inhibitor 16/PI16

PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator. Mature mouse PI16 is a type I transmembrane protein 469 amino acids (aa) in length. The extracellular region includes all but the C‑terminal 21 amino acids. A soluble form of PI16 is known. Although its predicted MW is 50 kDa, it runs anomalously at 70 kDa (unmodified) in SDS-PAGE. With glycosylation, it appears to be 100‑110 kDa in size. Three potential variants exist. One shows a deletion of aa 51‑124, a second shows a 9 aa extension at the N‑terminus, and a third shows a deletion of aa 191‑452. Over aa 1‑464, mouse PI16 shares 86% and 61% aa identity with rat and human PI16, respectively.

Alternate Names

CRISP9, MSMBBP, PI16, PSPBP

Entrez Gene IDs

221476 (Human); 74116 (Mouse); 294312 (Rat)

Gene Symbol

PI16

UniProt

Q9ET66

Additional Peptidase Inhibitor 16/PI16 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Cancer Biomarkers

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