Plasminogen is a coenzyme that has five triple disulfide-linked kringle domains. It is converted to plasmin by plasminogen activators. Internal fragments of plasminogen, including the naturally occurring angiostatin isoforms and Kringle 5, can also be generated by proteolytic cleavage. These internal fragments have been shown to be potent angiogenesis inhibitors (1).
Mouse Plasminogen Kringle 5 Antibody (120304)
R&D Systems | Catalog # MAB742
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
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Applications
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Cited:
Label
Antibody Source
Product Specifications
Immunogen
Cys481-Ala563
Accession # AAA50168
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse Plasminogen Kringle 5 Antibody (120304)
Detection of Mouse Plasminogen Kringle 5 by Western Blot
Ferulic acid regulated the differential expression of angiogenic and antiangiogenic factors in MN mouse model. At the end of the experiment, kidney samples of different groups were harvested and freshly isolated the glomeruli according to the Materials and Methods. Total protein lysate was extracted from isolated glomeruli and separated in 10% SDS page by electrophoresis, and further blotting by anti-VEGF (a), TSP-1 (b), and K5 (c) antibodies. The bar chart represented the semiquantification level of the expression density (target/actin) (Left Panel) (*P < 0.05; **P < 0.01). Immunofluorescence staining was also undertaken to exam the expression level and location of VEGF, TSP-1, and K5 in NC and N-T, original magnifications, 400x (Right Panel). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22844329), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse Plasminogen Kringle 5 Antibody (120304)
Western Blot
Sample: Recombinant Mouse Plasminogen Kringle 5
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Plasminogen Kringle 5
References
- Cao, Y. et al. (1997) J. Biol. Chem. 272:22924.
Alternate Names
Gene Symbol
UniProt
Additional Plasminogen Kringle 5 Products
Product Documents for Mouse Plasminogen Kringle 5 Antibody (120304)
Certificate of Analysis
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Product Specific Notices for Mouse Plasminogen Kringle 5 Antibody (120304)
For research use only
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Citations for Mouse Plasminogen Kringle 5 Antibody (120304)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
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