Mouse Protein S/PROS1 Antibody (818002)

R&D Systems | Catalog # MAB4976

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 818002
View all Protein S/PROS1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Protein S/PROS1
Met1-Arg669
Accession # Q08761

Specificity

Detects mouse Protein S/PROS1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Protein S is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Protein S/PROS1 Antibody (818002)

Detection of Mouse Protein S/PROS1 antibody by Western Blot.

Detection of Mouse Protein S/PROS1 by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Protein S/PROS1 Monoclonal Antibody (Catalog # MAB4976) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein S/PROS1 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Protein S/PROS1 Antibody (818002)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein S/PROS1

Protein S (PROS1) is a 70-75 kDa plasma glycoprotein member of the vitamin K dependent family of proteins.  It is synthesized by hepatocytes, Sertoli cells, macrophages, vascular smooth muscle cells and endothelium.  Protein S is a non-enzymatic cofactor for activated protein C and participates in the degradation of coagulation factors FVa and FVIIIa.  It also binds to Axl on smooth muscle, likely initiating mitosis.  Protein S circulates free in plasma and, unlike Protein S in human and rat, does not complex with C4BP.  Mouse Protein S precursor is 675 amino acids (aa) in length.  It contains a signal sequence (aa 1-24), a cleavable prosegment (aa 25-41) and a 634 aa mature region.  The mature molecule contains one Gla region (aa 42-87), four EGF-like domains (aa 117-283) and two laminin G-like regions (aa 299-665).  Over aa 1-169 of the mature molecule, mouse Protein S shares 80% and 88% aa identity with human and rat Protein S, respectively.

Alternate Names

PROS1

Entrez Gene IDs

5627 (Human); 19128 (Mouse); 81750 (Rat)

Gene Symbol

PROS1

UniProt

Q08761

Additional Protein S/PROS1 Products

Product Documents for Mouse Protein S/PROS1 Antibody (818002)

Certificate of Analysis

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Product Specific Notices for Mouse Protein S/PROS1 Antibody (818002)

For research use only

Citations for Mouse Protein S/PROS1 Antibody (818002)

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FAQs

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Associated Pathways

Blood Coagulation Signaling Pathways
Blood Coagulation Signaling Pathway Thumbnail