Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HIN-1/SCGB3A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse HIN‑1/SCGB3A1
Phe22-Gly104
Accession # Q920D7

Specificity

Detects mouse HIN‑1/SCGB3A1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human HIN-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat HIN‑1/SCGB3A1 Antibody

Detection of Rat HIN-1 antibody by Western Blot.

Detection of Rat HIN-1 by Western Blot.

Western blot shows lysates of rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat HIN-1/SCGB3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIN-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse/Rat HIN‑1/SCGB3A1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Rat lung tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HIN-1/SCGB3A1

HIN-1, also known as PnSP‑2, LuLeu‑2, UGRP‑2 and SCGB3A1, is an 10 kDa member of the secretoglobulin family of molecules. It is synthesized as a 104 amino acid (aa) precursor that contains a 21 aa signal sequence and an 83 aa mature region. There are no potential N‑linked glycosylation sites. Based on other members of the family, HIN‑1 is likely to exist as a disulfide‑linked homodimer. The molecule is found in mammary and upper airway epithelium. No receptor for HIN‑1 has been identified. Mature mouse HIN‑1 shares 60% aa sequence identity with mature human HIN‑1.

Long Name

High in Normal 1

Alternate Names

HIN1, PnSP-2, SCGB3A1, UGRP2

Entrez Gene IDs

92304 (Human); 73945 (Mouse)

Gene Symbol

SCGB3A1

UniProt

Q920D7

Additional HIN-1/SCGB3A1 Products

Product Documents for Mouse/Rat HIN‑1/SCGB3A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat HIN‑1/SCGB3A1 Antibody

For research use only

Citations for Mouse/Rat HIN‑1/SCGB3A1 Antibody

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