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Mouse/Rat HIN-1/SCGB3A1 Antibody

Catalog #: AF2954
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2954
AF2954-SP

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Detection of Rat HIN-1 by Western Blot.
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Citations (3)
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Mouse/Rat HIN-1/SCGB3A1 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse HIN‑1/SCGB3A1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human HIN-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse HIN‑1/SCGB3A1
Phe22-Gly104
Accession # Q920D7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat HIN-1 antibody by Western Blot. View Larger

Detection of Rat HIN-1 by Western Blot. Western blot shows lysates of rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat HIN-1/SCGB3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIN-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIN-1/SCGB3A1

HIN-1, also known as PnSP‑2, LuLeu‑2, UGRP‑2 and SCGB3A1, is an 10 kDa member of the secretoglobulin family of molecules. It is synthesized as a 104 amino acid (aa) precursor that contains a 21 aa signal sequence and an 83 aa mature region. There are no potential N‑linked glycosylation sites. Based on other members of the family, HIN‑1 is likely to exist as a disulfide‑linked homodimer. The molecule is found in mammary and upper airway epithelium. No receptor for HIN‑1 has been identified. Mature mouse HIN‑1 shares 60% aa sequence identity with mature human HIN‑1.

Long Name
High in Normal 1
Entrez Gene IDs
92304 (Human); 73945 (Mouse)
Alternate Names
cytokine high in normal-1; Cytokine HIN-1; High in normal 1; HIN1; HIN-1; HIN1Pneumo secretory protein 2; LU105; PNSP2; PnSP-2; PnSP-2Uteroglobin-related protein 2; SCGB3A1; secretoglobin family 3A member 1; secretoglobin, family 3A, member 1; UGRP2; UGRP2MGC87867

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Citations for Mouse/Rat HIN-1/SCGB3A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Neonatal Hyperoxic Exposure Persistently Alters Lung Secretoglobins and Annexin A1
    Authors: Thomas M. Raffay, Morgan L. Locy, Cynthia L. Hill, Nik S. Jindal, Lynette K. Rogers, Stephen E. Welty et al.
    BioMed Research International
  2. Spatiotemporal Expression of Three Secretoglobin Proteins, SCGB1A1, SCGB3A1, and SCGB3A2, in Mouse Airway Epithelia
    Authors: Xu Naizhen, Taketomo Kido, Shigetoshi Yokoyama, R. Ilona Linnoila, Shioko Kimura
    Journal of Histochemistry & Cytochemistry
  3. IFN-gamma down-regulates Secretoglobin 3A1 gene expression.
    Authors: Yamada A, Suzuki D, Miyazono A, Oshima K, Kamiya A, Zhao B, Takami M, Donnelly RP, Itabe H, Yamamoto M, Kimura S, Kamijo R
    Biochem. Biophys. Res. Commun., 2009-01-09;379(4):964-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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