PDZK1 Antibody

R&D Systems | Catalog # AF5780

R&D Systems

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PDZK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse PDZK1
aa 1-519
Accession # Q9JIL4

Specificity

Detects mouse and rat PDZK1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PDZK1 Antibody

Detection of Mouse and Rat PDZK1 antibody by Western Blot.

Detection of Mouse and Rat PDZK1 by Western Blot.

Western blot shows lysates of mouse liver tissue and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PDZK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5780) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PDZK1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for PDZK1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse liver tissue and rat liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDZK1

PDZK1 (PDZ Domain-containing 1; also CFTR-associated protein of 70 kDa/CAP70) is a 65‑70 kDa member of the NHER (Na/H Exchange Regulator) family of proteins. It is only expressed in epithelium, particularly that found in intestine, kidney and liver. PDZK1 serves as a scaffold protein that connects cell membrane proteins to cytoplasmic regulators. It also serves a critical role in basal-level bicarbonate secretion. Mouse PDZK1 is 519 amino acids (aa) in length and contains four PDZ domains (aa 9‑90; 128‑215; 243‑323; and 378‑458) plus three phosphorylation sites at Ser489, Ser492 and Ser514. Mouse PDZK1 shares 78% and 91% aa identity with human and rat PDZK1, respectively.

Long Name

PDZ Domain Containing 1

Alternate Names

CAP70, CLAMP, NaPi-Cap1, NHERF3, PDZD1

Entrez Gene IDs

5174 (Human); 59020 (Mouse); 65144 (Rat)

Gene Symbol

PDZK1

UniProt

Q9JIL4

Additional PDZK1 Products

Product Documents for PDZK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for PDZK1 Antibody

For research use only

Related Research Areas

Epithelial Cell Polarity

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