Thioredoxin-like 5/TRP14 Antibody

R&D Systems | Catalog # AF3769

R&D Systems

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Thioredoxin-like 5/TRP14 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Thioredoxin-like 5/TRP14
Met1-Asp123
Accession # Q9CQM5

Specificity

Detects endogenous mouse and rat Thioredoxin-like 5/TRP14 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Thioredoxin-like 5/TRP14 Antibody

Detection of Mouse/Rat Thioredoxin-like 5/TRP14 antibody by Western Blot.

Detection of Mouse/Rat Thioredoxin-like 5/TRP14 by Western Blot.

Western blot shows lysates of A20 mouse B cell lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat Thioredoxin-like 5/TRP14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3769) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Thioredoxin-like 5/TRP14 at approximately 14 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Applications for Thioredoxin-like 5/TRP14 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: A20 mouse B cell lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thioredoxin-like 5/TRP14

Thioredoxin-like 5, also known as a 14 kDa thioredoxin-related protein (TRP14) is a 14 kDa cytosolic redox regulatory molecule that belongs to the thioredoxin family. It contains a thioredoxin-like redox-sensitive active site motif (Cys-Pro-Asp-Cys). In its reduced form, the active site cysteine residues reduce protein disulfide bonds. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by the NADPH-dependent thioredoxin reductase 1. Thioredoxin-like 5 is ubiquitously expressed and acts on protein targets different from that of Thioredoxin-1. The amino acid sequence of mouse Thioredoxin-like 5 is 96% identical to that of rat Thioredoxin-like 5.

Alternate Names

Thioredoxin like 5, TRP14, TXNDC17, TXNL5

Entrez Gene IDs

84817 (Human); 52700 (Mouse)

Gene Symbol

TXNDC17

UniProt

Q9CQM5

Additional Thioredoxin-like 5/TRP14 Products

Product Documents for Thioredoxin-like 5/TRP14 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Thioredoxin-like 5/TRP14 Antibody

For research use only

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