VSIG1 Antibody

R&D Systems | Catalog # AF5324

R&D Systems

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VSIG1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VSIG1
Val23-Glu234
Accession # BAE35812

Specificity

Detects mouse and rat VSIG1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) VSIG1 is observed and less than 1% cross‑reactivity with recombinant mouse (rm) VSIG2, rmVSIG3, rmVSIG4, and rhVSIG3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VSIG1 Antibody

Detection of Mouse and Rat VSIG1 antibody by Western Blot.

Detection of Mouse and Rat VSIG1 by Western Blot.

Western blot shows lysates of rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat VSIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5324) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for VSIG1 at approximately 55 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for VSIG1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Rat testis tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG1

VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein 34 and Cell surface antigen 33) is a 55‑70 kDa member of the JAM family of Ig-Superfamily proteins. It shows expression on gastric epithelium and testicular germ cells and likely participates in cell adhesion. Mouse VSIG1 precursor is 407 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 211 aa extracellular domain (ECD) (aa 23‑234) and a 152 aa cytoplasmic region. The ECD contains one V-type (aa 23‑134) and one C2-type Ig-like domain (aa 145‑229). Over aa 23‑234, mouse VSIG1 is 94% and 81% aa identical to rat and human VSIG1, respectively. There is one potential alternate start site at Met108.

Long Name

V-Set and Immunoglobulin Domain Containing 1

Alternate Names

GPA34

Entrez Gene IDs

340547 (Human); 78789 (Mouse); 315920 (Rat); 102123142 (Cynomolgus Monkey)

Gene Symbol

VSIG1

UniProt

BAE35812

Additional VSIG1 Products

Product Documents for VSIG1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for VSIG1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Inflammatory Mediators

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