Reg2 is a secreted C-type lectin that is expressed in regenerating pancreatic islets and stimulates the growth of pancreatic beta cells.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 239237
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Reg2
Specificity
Detects mouse Reg2 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) Reg1, recombinant rat (rr) Reg2, rmReg3A, recombinant human Reg4, or rrReg4.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Reg2 Antibody
Detection of Mouse Reg2 by Western Blot.
Western blot shows lysates of NS0 mouse myeloma cell line, mouse small intestine and pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Reg2 Monoclonal Antibody (Catalog # MAB2098) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Reg2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: NS0 mouse myeloma cell line, mouse small intestine and pancreas tissue
Sample: NS0 mouse myeloma cell line, mouse small intestine and pancreas tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Reg2
Long Name
Regenerating Islet-derived 2
Alternate Names
regenerating islet-derived 2, RegII
Entrez Gene IDs
19693 (Mouse)
Gene Symbol
REG2
Additional Reg2 Products
Product Documents for Mouse Reg2 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Reg2 Antibody
For research use only
Related Research Areas
Citations for Mouse Reg2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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