Mouse S5a/Angiocidin Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5540R Datasheet / COA / SDS

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AF5540R-100UG

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Mouse S5a/Angiocidin Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse S5a/Angiocidin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse S5a/Angiocidin isoform A
Val2-Glu254
Accession # O35226

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: S5a/Angiocidin

Angiocidin (also S5a, 26S proteasome regulatory subunit S5A, Psmd4, RPN10a and Mcb1) is a 41 kDa (predicted) member of the proteosome subunit S5A family of proteins. It runs anomalously in SDS-PAGE at 55 kDa. Intracellularly, Angiocidin is a component of the 26S proteosome that degrades ubiquitinated proteins. Extracellularly, it inhibits angiogenesis by binding to collagen and beta 1 integrins. Mouse Angiocidin is 376 amino acids (aa) in length. It contains a vWF-A domain (aa 5‑188), a beta 1 interacting site (aa 86‑105) and two ubiquitin-interacting motifs (aa 211‑230 and 282‑301). There are four splice variants. Three show a GlyGluArg insertion after Glu254, with two of these showing an additional three aa substitution for aa 255‑376, and a 45 aa substitution for aa 321‑376, respectfully. A fourth shows a 51 aa substitution for aa 299‑376. Over aa 1‑254, mouse Angiocidin shares > 99% aa identity with rat and human Angiocidin.

Long Name

26S Proteasome Regulatory Subunit S5a

Entrez Gene IDs

5710 (Human); 19185 (Mouse); 83499 (Rat)

Alternate Names

26S proteasome non-ATPase regulatory subunit 4;AF;AF-1;ASF;MCB1;PSMD4;pUB-R5;Rpn10;S5A; Angiocidin; ASF; Macropain; Mcb1; PSMD4; pUB-R5; Rpn10 ; S5a