Mouse Serpin A3N Alexa Fluor® 647-conjugated Antibody

Catalog #: AF4709R Datasheet / COA / SDS

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AF4709R-100UG

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All Serpin A3N Antibodies

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Mouse Serpin A3N Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin A3N in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Serpin A1, recombinant human Serpin A3 and rmSerpin A5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin A3N
Phe21-Lys418
Accession # Q91WP6

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Serpin A3N

Serpin A3N is a serine protease inhibitor that is structurally related to alpha 1-antichymotrypsin encoded by the SERPINA3 gene (1). Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen (2). Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B (3). Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme B-mediated killing by cytotoxic lymphocytes, providing a means to disable cell-mediated immune responses.

Entrez Gene IDs

20716 (Mouse); 24795 (Rat)

Alternate Names

A1A;A1AT;AAT;Alpha-1-antitrypsin;alpha1AT;nNIF;PI;PI1;PRO2275;SERPINA1; Serpin A3N; Spi2