Mouse Siglec-1/CD169 Antibody

R&D Systems | Catalog # AF5610

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Siglec-1/CD169 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Siglec‑1/CD169
Thr20-Leu1639
Accession # Q62230

Specificity

Detects mouse Siglec‑1/CD169 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) Siglec-1 is observed and less than 1% cross-reactivity with rhSiglec-2 and recombinant mouse Siglec-2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse Siglec-1/CD169 Antibody

Detection of Mouse Siglec-1/CD169 antibody by Western Blot.

Detection of Mouse Siglec‑1/CD169 by Western Blot.

Western blot shows lysates of mouse lymph node tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Siglec-1/CD169 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5610) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Siglec-1/CD169 at approximately 180-190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cell Adhesion Mediated by Siglec‑1/CD169 and Neutral-ization by Mouse Siglec‑1/CD169 Antibody.

Cell Adhesion Mediated by Siglec‑1/CD169 and Neutral-ization by Mouse Siglec‑1/CD169 Antibody.

Cell Adhesion Mediated by Siglec-1/CD169 and Neutralization by Mouse Siglec-1/CD169 Antibody. Recombinant Mouse Siglec-1/CD169 Fc Chimera (Catalog # 5610-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Siglec-1/CD169 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse Siglec-1/CD169 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5610). The ND50 is typically 1-5 µg/mL.

Applications for Mouse Siglec-1/CD169 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse lymph node tissue

Neutralization

Measured by its ability to neutralize Siglec‑1/CD169-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Mouse Siglec‑1/CD169 Fc Chimera.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-1/CD169

Siglecs are sialic acid specific I-type lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N-terminal Ig-like V‑set domain followed by varying numbers of Ig-like C2-set domains (1, 2). Mouse Siglec-1, also known as sialoadhesin and CD169, is a 175‑185 kDa glycoprotein that consists of a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V‑set domain and 16 Ig-like C2-set domains, a 21 aa transmembrane segment, and a 35 aa cytoplasmic domain (3, 4). Within the ECD, mouse Siglec-1 shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates a soluble form of the ECD and a soluble isoform that is truncated following the first three Ig-like domains (3). Siglec-1 expression is restricted to lymph node and spleen macrophages and some tissue macrophages (4). The adhesive function of Siglec-1 is supported by the N-terminal Ig-like domain which shows a selectivity for alpha -2,3-linked sialic acid residues (4‑6). Siglec-1 binds a number of sialylated molecules including the mannose receptor, MGL1, MUC1, PSGL-1, and different glycoforms of CD43 (7‑10). Its binding capacity can be masked by endogenous sialylated molecules (11, 12). The sialylated and sulfated N-linked carbohydrates that modify Siglec-1 itself are required for ligand binding (7, 8). Siglec-1 is expressed on dendritic cells following rhinovirus exposure, and these DC promote T cell anergy (13). It is also induced on circulating monocytes during systemic sclerosis and HIV‑1 infection (14‑16). Siglec-1 can trap HIV‑1 particles for trans-infection of permissive cells (15).

References

  1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
  2. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
  3. Crocker, P.R. et al. (1994) EMBO J. 13:4490.
  4. Hartnell, A. et al. (2001) Blood 97:288.
  5. Nath, D. et al. (1995) J. Biol. Chem. 270:26184.
  6. Crocker, P.R. et al. (1991) EMBO J. 10:1661.
  7. Martinez-Pomares, L. et al. (1999) J. Biol. Chem. 274:35211.
  8. Kumamoto, Y. et al. (2004) J. Biol. Chem. 279:49274.
  9. Nath, D. et al. (1999) Immunology 98:213.
  10. van den Berg, T.K. et al. (2001) J. Immunol. 166:3637.
  11. Nakamura, K. et al. (2002) Glycobiology 12:209.
  12. Barnes, Y.C. et al. (1999) Blood 93:1245.
  13. Kirchberger, S. et al. (2005) J. Immunol. 175:1145.
  14. York, M.R. et al. (2007) Arthritis Rheum. 56:1010.
  15. Rempel, H. et al. (2008) PloS ONE 3:e1967.
  16. van der Kuyl, A.C. et al. (2007) Plos ONE 2:e257.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Entrez Gene IDs

6614 (Human); 20612 (Mouse); 311426 (Rat)

Gene Symbol

SIGLEC1

UniProt

Q62230

Additional Siglec-1/CD169 Products

Product Documents for Mouse Siglec-1/CD169 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Siglec-1/CD169 Antibody

For research use only

Citations for Mouse Siglec-1/CD169 Antibody

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