Mouse Sulfatase Modifying Factor 1/SUMF1 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF3335V Datasheet / COA / SDS

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AF3335V-100UG

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All Sulfatase Modifying Factor 1/SUMF1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Sulfatase Modifying Factor 1/SUMF1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Sulfatase Modifying Factor 1/SUMF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human SUMF1 is observed and 10% cross-reactivity with recombinant mouse

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Sulfatase Modifying Factor 1/SUMF1
Ser32-Asp372
Accession # Q8R0F3

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Sulfatase Modifying Factor 1/SUMF1

Mammalian sulfatases have a common CXPXR motif, in which the Cys residue is post-translationally modified to a Calpha-formylglycine (FGly) residue. This modification is essential for catalytic activity of all sulfatases and is catalyzed by FGly generating enzyme (FGE) encoded by sulfatase modifying factor 1 (SUMF1). FGE deficiency causes multiple sulfatase deficiency (MSD), a rare autosomal recessive disorder. SUMF2 does not have FGly enzymatic activity, but may compete with SUMF1 for the same substrate.

Entrez Gene IDs

285362 (Human); 58911 (Mouse); 362409 (Rat)

Alternate Names

AAPA3037; EC 1.8.99.-; FGE1; FGEC-alpha-formylglycine-generating enzyme 1; FGly-generating enzyme; MGC131853; MGC150436; sulfatase modifying factor 1; sulfatase-modifying factor 1; SUMF1; UNQ3037